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Chlorine in PDB 7o5l: Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations

Enzymatic activity of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations

All present enzymatic activity of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations:
3.3.1.1;

Protein crystallography data

The structure of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations, PDB code: 7o5l was solved by P.H.Malecki, B.Imiolczyk, J.Barciszewski, J.Czyrko-Horczak, K.Brzezinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.63 / 1.74
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 120.77, 196.07, 82.052, 90, 90, 90
R / Rfree (%) 18.8 / 21.4

Other elements in 7o5l:

The structure of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations also contains other interesting chemical elements:

Rubidium (Rb) 6 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations (pdb code 7o5l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations, PDB code: 7o5l:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in 7o5l

Go back to Chlorine Binding Sites List in 7o5l
Chlorine binding site 1 out of 4 in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl506

b:47.9
occ:0.50
CL A:CL506 0.0 47.9 0.5
CL A:CL506 2.0 47.4 0.5
NE A:ARG41 3.2 34.8 1.0
CD A:ARG41 4.0 36.8 1.0
NH2 A:ARG41 4.1 38.4 1.0
CG A:GLN37 4.1 44.2 1.0
CZ A:ARG41 4.1 40.4 1.0
CG1 A:ILE38 4.1 29.1 1.0
NE2 A:GLN37 4.1 51.3 1.0
CA A:ILE38 4.4 29.0 1.0
O A:GLN37 4.4 33.0 1.0
CD A:GLN37 4.5 48.0 1.0
N A:ILE38 4.5 29.3 1.0
C A:GLN37 4.6 31.5 1.0
CB A:ARG41 4.6 35.2 1.0
CB A:ILE38 4.9 25.9 1.0
CB A:GLN37 4.9 32.0 1.0
O A:HOH878 5.0 48.8 1.0
CG A:ARG41 5.0 32.8 1.0

Chlorine binding site 2 out of 4 in 7o5l

Go back to Chlorine Binding Sites List in 7o5l
Chlorine binding site 2 out of 4 in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl506

b:47.4
occ:0.50
CL A:CL506 0.0 47.4 0.5
CL A:CL506 2.0 47.9 0.5
NH2 A:ARG41 3.6 38.4 1.0
NE A:ARG41 3.8 34.8 1.0
CZ A:ARG41 4.1 40.4 1.0
O A:HOH878 4.1 48.8 1.0
CD2 A:LEU368 4.3 29.8 1.0
CG1 A:ILE38 4.4 29.1 1.0
CD1 A:LEU368 4.4 25.4 1.0
CD A:ARG41 4.9 36.8 1.0

Chlorine binding site 3 out of 4 in 7o5l

Go back to Chlorine Binding Sites List in 7o5l
Chlorine binding site 3 out of 4 in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl505

b:51.6
occ:0.40
CL C:CL505 0.0 51.6 0.4
CL C:CL505 1.8 49.8 0.4
O C:HOH849 3.0 43.3 0.5
O C:HOH652 3.2 49.2 1.0
O C:HOH849 3.3 47.3 0.5
NE C:ARG41 3.3 51.8 1.0
O C:HOH644 3.5 50.6 1.0
NH2 C:ARG41 4.0 53.5 1.0
CD C:ARG41 4.1 55.2 1.0
CZ C:ARG41 4.1 56.6 1.0
CG1 C:ILE38 4.4 45.9 1.0
CA C:ILE38 4.7 44.0 1.0
CB C:ARG41 4.9 51.6 1.0
CD1 C:LEU398 4.9 36.9 1.0
N C:ILE38 4.9 48.4 1.0

Chlorine binding site 4 out of 4 in 7o5l

Go back to Chlorine Binding Sites List in 7o5l
Chlorine binding site 4 out of 4 in the Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl505

b:49.8
occ:0.40
CL C:CL505 0.0 49.8 0.4
CL C:CL505 1.8 51.6 0.4
O C:HOH652 3.4 49.2 1.0
NH2 C:ARG41 3.6 53.5 1.0
NE C:ARG41 3.9 51.8 1.0
CG1 C:ILE38 4.1 45.9 1.0
CZ C:ARG41 4.2 56.6 1.0
CD1 C:LEU368 4.2 40.4 1.0
CD2 C:LEU368 4.3 46.5 1.0
O C:HOH849 4.7 47.3 0.5
O C:HOH849 4.7 43.3 0.5
CD1 C:ILE38 4.8 37.5 1.0
O C:HOH644 4.8 50.6 1.0
CD1 C:LEU398 4.9 36.9 1.0
CG C:LEU368 4.9 38.5 1.0
O C:HOH804 4.9 50.4 1.0

Reference:

P.H.Malecki, B.Imiolczyk, J.Barciszewski, J.Czyrko-Horczak, J.Sliwiak, M.Gawel, K.Wozniak, M.Jaskolski, K.Brzezinski. Biochemical and Structural Insights Into An Unusual, Alkali-Metal-Independent S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803. Acta Crystallogr D Struct V. 78 865 2022BIOL.
ISSN: ISSN 2059-7983
PubMed: 35775986
DOI: 10.1107/S2059798322005605
Page generated: Sun Jul 13 04:43:08 2025

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