Atomistry » Chlorine » PDB 7p2u-7pe4 » 7pcw
Atomistry »
  Chlorine »
    PDB 7p2u-7pe4 »
      7pcw »

Chlorine in PDB 7pcw: X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate

Enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate

All present enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate, PDB code: 7pcw was solved by M.Tarnawski, M.Frei, J.Hiblot, K.Johnsson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.36 / 2.30
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 44.32, 76.56, 85.76, 68.4, 79.81, 79.02
R / Rfree (%) 19.1 / 24.3

Chlorine Binding Sites:

The binding sites of Chlorine atom in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate (pdb code 7pcw). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate, PDB code: 7pcw:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in 7pcw

Go back to Chlorine Binding Sites List in 7pcw
Chlorine binding site 1 out of 4 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl302

b:22.2
occ:1.00
NE1 A:TRP107 3.3 24.0 1.0
ND2 A:ASN41 3.3 18.5 1.0
N A:PRO206 3.4 20.3 1.0
CA A:PRO206 3.6 22.4 1.0
C A:PHE205 3.7 23.9 1.0
CD A:PRO206 3.7 19.4 1.0
CD1 A:TRP107 3.8 20.1 1.0
CB A:PHE205 3.8 23.6 1.0
CZ A:PHE168 3.9 26.2 1.0
C20 A:OEH301 3.9 25.7 1.0
CB A:ASN41 3.9 23.0 1.0
CB A:PRO206 3.9 19.9 1.0
O A:PHE205 4.0 20.8 1.0
CD1 A:LEU209 4.0 22.1 1.0
CD2 A:LEU209 4.1 18.4 1.0
CG A:ASN41 4.1 22.1 1.0
CG A:LEU209 4.1 24.9 1.0
C19 A:OEH301 4.2 27.4 1.0
C18 A:OEH301 4.3 21.5 1.0
CA A:PHE205 4.4 25.2 1.0
CG A:PRO206 4.4 20.0 1.0
CE2 A:TRP107 4.5 23.4 1.0
CE2 A:PHE168 4.5 20.4 1.0
CE2 A:PHE149 4.7 26.6 1.0
CE1 A:PHE168 4.7 20.3 1.0
OD1 A:ASP106 4.7 27.1 1.0
CG A:PHE205 4.8 23.5 1.0

Chlorine binding site 2 out of 4 in 7pcw

Go back to Chlorine Binding Sites List in 7pcw
Chlorine binding site 2 out of 4 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl302

b:20.8
occ:1.00
NE1 B:TRP107 3.2 24.2 1.0
N B:PRO206 3.4 19.1 1.0
ND2 B:ASN41 3.5 19.4 1.0
CA B:PRO206 3.6 23.7 1.0
CD1 B:TRP107 3.6 22.7 1.0
C B:PHE205 3.7 22.1 1.0
CD B:PRO206 3.8 17.9 1.0
CB B:PRO206 3.9 24.4 1.0
C20 B:OEH301 3.9 23.0 1.0
CD1 B:LEU209 3.9 20.3 1.0
CB B:PHE205 3.9 19.5 1.0
CZ B:PHE168 4.0 16.1 1.0
CB B:ASN41 4.0 24.5 1.0
O B:PHE205 4.0 20.2 1.0
CG B:LEU209 4.0 21.2 1.0
CD2 B:LEU209 4.1 19.8 1.0
C19 B:OEH301 4.1 25.4 1.0
C18 B:OEH301 4.2 29.7 1.0
CG B:ASN41 4.3 22.4 1.0
CE2 B:TRP107 4.4 24.8 1.0
CG B:PRO206 4.4 23.0 1.0
CA B:PHE205 4.5 21.4 1.0
OD1 B:ASP106 4.6 26.3 1.0
CE2 B:PHE168 4.6 22.2 1.0
CE2 B:PHE149 4.7 17.1 1.0
CE1 B:PHE168 4.8 19.8 1.0
CG B:PHE205 4.9 25.5 1.0
CG B:TRP107 5.0 20.3 1.0
OD2 B:ASP106 5.0 27.9 1.0

Chlorine binding site 3 out of 4 in 7pcw

Go back to Chlorine Binding Sites List in 7pcw
Chlorine binding site 3 out of 4 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl302

b:21.4
occ:1.00
NE1 C:TRP107 3.3 28.2 1.0
ND2 C:ASN41 3.4 22.3 1.0
N C:PRO206 3.4 21.9 1.0
CA C:PRO206 3.6 19.8 1.0
C C:PHE205 3.7 24.0 1.0
CD1 C:TRP107 3.8 24.1 1.0
CD C:PRO206 3.8 17.1 1.0
CB C:PRO206 3.9 16.0 1.0
CD1 C:LEU209 3.9 23.5 1.0
C20 C:OEH301 3.9 32.6 1.0
CZ C:PHE168 3.9 24.3 1.0
CB C:PHE205 3.9 26.8 1.0
CB C:ASN41 4.0 21.5 1.0
O C:PHE205 4.0 27.0 1.0
CG C:LEU209 4.1 27.3 1.0
CD2 C:LEU209 4.2 21.3 1.0
CG C:ASN41 4.2 27.1 1.0
C18 C:OEH301 4.2 29.0 1.0
C19 C:OEH301 4.3 33.2 1.0
CG C:PRO206 4.4 22.3 1.0
CA C:PHE205 4.5 25.6 1.0
CE2 C:PHE168 4.5 19.5 1.0
CE2 C:TRP107 4.5 28.6 1.0
OD1 C:ASP106 4.6 31.2 1.0
CE2 C:PHE149 4.8 17.1 1.0
CE1 C:PHE168 4.8 16.9 1.0
CG C:PHE205 4.9 24.7 1.0
C C:PRO206 5.0 25.5 1.0

Chlorine binding site 4 out of 4 in 7pcw

Go back to Chlorine Binding Sites List in 7pcw
Chlorine binding site 4 out of 4 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl302

b:25.1
occ:1.00
NE1 D:TRP107 3.0 26.0 1.0
N D:PRO206 3.4 22.9 1.0
ND2 D:ASN41 3.5 24.5 1.0
CD1 D:TRP107 3.6 26.0 1.0
CA D:PRO206 3.6 23.4 1.0
C D:PHE205 3.6 24.8 1.0
C20 D:OEH301 3.7 34.6 1.0
CB D:PHE205 3.8 22.6 1.0
O D:PHE205 3.8 24.6 1.0
CB D:PRO206 3.9 16.6 1.0
CD D:PRO206 3.9 22.3 1.0
CB D:ASN41 4.0 26.4 1.0
CD1 D:LEU209 4.0 24.9 1.0
CG D:LEU209 4.1 23.4 1.0
CD2 D:LEU209 4.1 20.9 1.0
CZ D:PHE168 4.2 32.6 1.0
C19 D:OEH301 4.2 28.5 1.0
CE2 D:TRP107 4.2 27.3 1.0
C18 D:OEH301 4.2 30.3 1.0
CG D:ASN41 4.3 29.7 1.0
CA D:PHE205 4.3 23.9 1.0
CG D:PRO206 4.5 20.7 1.0
OD1 D:ASP106 4.6 25.9 1.0
CG D:PHE205 4.7 25.0 1.0
CE2 D:PHE168 4.8 28.3 1.0
CZ2 D:TRP107 4.8 22.9 1.0
CG D:TRP107 4.9 19.9 1.0
CE1 D:PHE168 4.9 25.8 1.0
CE2 D:PHE149 4.9 24.3 1.0
OD2 D:ASP106 4.9 31.6 1.0

Reference:

M.Tarnawski, M.Frei, J.Hiblot, K.Johnsson. X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7-M175W Labeled with A Chloroalkane-Tetramethylrhodamine Fluorophore Substrate To Be Published.
Page generated: Sun Jul 13 05:36:45 2025

Last articles

Mg in 7FS2
Mg in 7FS1
Mg in 7FS0
Mg in 7FRZ
Mg in 7FRY
Mg in 7FRX
Mg in 7FRW
Mg in 7FRV
Mg in 7FJP
Mg in 7FQJ
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy