Chlorine in PDB 7z21: Baf A12T Bound to the Lamin A/C Ig-Fold Domain

The structure of Baf A12T Bound to the Lamin A/C Ig-Fold Domain, PDB code: 7z21 was solved by A.Marcelot, P.Legrand, S.Zinn-Justin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.90 / 1.63
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.11, 75.073, 65.034, 90, 114.19, 90
R / Rfree (%)	26.8 / 30

The binding sites of Chlorine atom in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain (pdb code 7z21). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain, PDB code: 7z21:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Baf A12T Bound to the Lamin A/C Ig-Fold Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl101 b:30.3 occ:1.00 H A:GLY25 2.2 9.1 1.0 HG A:SER4 2.2 15.6 1.0 O A:HOH301 2.8 26.9 1.0 HA A:ALA24 3.0 9.0 1.0 HB3 A:LYS6 3.2 17.0 1.0 OG A:SER4 3.2 15.6 1.0 HD2 A:LYS6 3.2 25.3 1.0 N A:GLY25 3.2 9.1 1.0 O A:HOH213 3.3 22.1 1.0 HB3 A:SER4 3.5 15.6 1.0 O A:HOH305 3.5 27.8 1.0 HB2 A:SER4 3.5 15.6 1.0 CB A:SER4 3.6 15.6 1.0 O A:HOH271 3.6 18.6 1.0 HB1 A:ALA24 3.7 9.1 1.0 HA2 A:GLY25 3.9 9.7 1.0 CA A:ALA24 3.9 9.0 1.0 HB2 A:LYS6 4.0 17.0 1.0 HE3 A:LYS6 4.0 26.9 1.0 C A:ALA24 4.0 8.8 1.0 CB A:LYS6 4.0 17.0 1.0 HZ2 A:LYS6 4.1 26.5 1.0 CA A:GLY25 4.1 9.7 1.0 CD A:LYS6 4.2 25.3 1.0 O A:HOH225 4.3 22.2 1.0 CB A:ALA24 4.3 9.2 1.0 CE A:LYS6 4.5 26.9 1.0 O A:HOH256 4.6 20.6 1.0 HB3 A:ASP76 4.6 10.7 1.0 H A:LYS6 4.6 14.6 1.0 O A:HOH309 4.6 28.0 1.0 H A:HIS7 4.6 11.3 1.0 O A:HOH300 4.7 37.8 1.0 CG A:LYS6 4.7 22.0 1.0 NZ A:LYS6 4.8 26.5 1.0 HB2 A:ALA24 4.8 9.2 1.0 OD2 A:ASP76 4.9 18.2 1.0 H A:ILE26 4.9 9.8 1.0 C A:GLY25 4.9 10.4 1.0 O E:HOH679 4.9 11.3 1.0 HD3 A:LYS6 5.0 25.3 1.0 HA3 A:GLY25 5.0 9.7 1.0

Chlorine binding site 2 out of 4 in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Baf A12T Bound to the Lamin A/C Ig-Fold Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl101 b:25.4 occ:1.00 H B:GLY25 2.4 9.9 1.0 HG B:SER4 2.4 15.6 1.0 HD3 B:LYS6 3.1 23.9 1.0 HB2 B:LYS6 3.2 16.1 1.0 HA B:ALA24 3.3 10.2 1.0 OG B:SER4 3.3 15.6 1.0 N B:GLY25 3.4 9.9 1.0 O B:HOH206 3.4 21.3 1.0 HE2 B:LYS6 3.5 25.1 1.0 O B:HOH266 3.5 20.7 1.0 HB3 B:SER4 3.5 15.2 1.0 HB2 B:SER4 3.5 15.2 1.0 CB B:SER4 3.7 15.2 1.0 HB1 B:ALA24 3.8 11.2 1.0 HA2 B:GLY25 3.9 10.3 1.0 CD B:LYS6 4.0 23.9 1.0 O B:HOH212 4.0 19.1 1.0 CA B:ALA24 4.1 10.2 1.0 CE B:LYS6 4.2 25.1 1.0 CA B:GLY25 4.2 10.2 1.0 HZ2 B:LYS6 4.2 24.6 1.0 CB B:LYS6 4.2 16.1 1.0 C B:ALA24 4.2 9.8 1.0 CB B:ALA24 4.5 11.0 1.0 HB3 B:LYS6 4.5 16.1 1.0 O B:HOH259 4.5 19.2 1.0 H B:LYS6 4.6 14.1 1.0 CG B:LYS6 4.7 20.4 1.0 HB3 B:ASP76 4.7 10.9 1.0 NZ B:LYS6 4.7 24.6 1.0 H B:HIS7 4.8 11.6 1.0 HD2 B:LYS6 4.8 23.9 1.0 HB2 B:ALA24 4.9 11.2 1.0 OD2 B:ASP76 4.9 17.4 1.0 C B:GLY25 5.0 11.0 1.0 HG3 B:LYS6 5.0 20.4 1.0

Chlorine binding site 3 out of 4 in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Baf A12T Bound to the Lamin A/C Ig-Fold Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl101 b:45.0 occ:1.00 HH22 C:ARG60 2.3 23.7 1.0 HH11 C:ARG75 2.4 34.9 1.0 HD3 C:ARG75 2.7 28.3 1.0 HH12 C:ARG60 2.7 23.3 1.0 NH2 C:ARG60 3.3 23.7 1.0 NH1 C:ARG75 3.3 34.9 1.0 NH1 C:ARG60 3.6 23.3 1.0 HH12 C:ARG75 3.8 34.9 1.0 CD C:ARG75 3.8 28.3 1.0 CZ C:ARG60 3.9 24.0 1.0 HB2 C:ARG75 4.0 19.3 1.0 HH21 C:ARG60 4.1 23.7 1.0 HD2 C:ARG75 4.2 28.3 1.0 HB1 C:ALA71 4.2 24.0 1.0 CZ C:ARG75 4.3 34.7 1.0 HH11 C:ARG60 4.4 23.3 1.0 NE C:ARG75 4.5 32.0 1.0 HG3 C:ARG75 4.5 23.6 1.0 CG C:ARG75 4.6 23.6 1.0 O C:HOH276 4.8 31.6 1.0 CB C:ARG75 4.8 19.3 1.0

Chlorine binding site 4 out of 4 in the Baf A12T Bound to the Lamin A/C Ig-Fold Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Baf A12T Bound to the Lamin A/C Ig-Fold Domain within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl101 b:45.0 occ:1.00 HH12 D:ARG60 2.5 23.5 1.0 HH22 D:ARG60 2.7 22.8 1.0 HD3 D:ARG75 3.2 28.0 1.0 NH1 D:ARG60 3.4 23.5 1.0 NH2 D:ARG60 3.6 22.8 1.0 CZ D:ARG60 4.0 23.7 1.0 HH11 D:ARG75 4.0 33.1 1.0 CD D:ARG75 4.2 28.0 1.0 HH11 D:ARG60 4.2 23.5 1.0 HD2 D:ARG75 4.3 28.0 1.0 HH21 D:ARG60 4.4 22.8 1.0 HB2 D:ARG75 4.5 19.9 1.0 HG3 D:ARG75 4.7 24.1 1.0 HB1 D:ALA71 4.7 24.1 1.0 CG D:ARG75 4.9 24.1 1.0 NH1 D:ARG75 5.0 33.1 1.0

A.Janssen, A.Marcelot, S.Breusegem, P.Legrand, S.Zinn-Justin, D.Larrieu. The Baf A12T Mutation Disrupts Lamin A/C Interaction, Impairing Robust Repair of Nuclear Envelope Ruptures in Nestor-Guillermo Progeria Syndrome Cells. Nucleic Acids Res. V. 50 9260 2022.
ISSN: ESSN 1362-4962
PubMed: 36039758
DOI: 10.1093/NAR/GKAC726