Chlorine in PDB 8b2d: Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+

All present enzymatic activity of Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+:
1.14.13.8;

The structure of Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+, PDB code: 8b2d was solved by I.Cea-Rama, J.Sanz-Aparicio, M.Ferrer Martinez, M.Goris, G.Bjerga, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.27 / 1.62
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	125.606, 130.361, 115.653, 90, 90, 90
R / Rfree (%)	16.2 / 18

The binding sites of Chlorine atom in the Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+ (pdb code 8b2d). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+, PDB code: 8b2d:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl508 b:49.0 occ:1.00 NH2 B:ARG244 3.7 30.4 1.0 NH1 B:ARG244 4.0 30.2 1.0 CB B:SER261 4.3 25.4 1.0 CZ B:ARG244 4.3 31.0 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Bacterial Flavin Containing Monooxygenase Thermoresistant Mutant, in Complex with Nadp+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl509 b:54.8 occ:1.00 NE B:ARG339 3.7 24.4 1.0 NH2 B:ARG339 3.8 24.2 1.0 CZ B:ARG339 4.2 23.1 1.0 CE B:MET337 4.4 24.2 1.0 CG B:ARG339 4.6 23.0 1.0 CB B:ALA29 4.7 22.6 1.0 CD B:ARG339 4.7 24.5 1.0

M.Goris, I.Cea-Rama, P.Puntervoll, R.Ree, D.Almendral, J.Sanz-Aparicio, M.Ferrer, G.E.K.Bjerga. Increased Thermostability of An Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates. Appl.Environ.Microbiol. 39023 2023.
ISSN: ESSN 1098-5336
PubMed: 37222584
DOI: 10.1128/AEM.00390-23