Chlorine in PDB 8e6i: X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State

The structure of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State, PDB code: 8e6i was solved by S.Ray, R.Gaudet, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.32 / 2.52
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	58.673, 71.35, 98.593, 90, 90, 90
R / Rfree (%)	22.5 / 26.6

The structure of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State also contains other interesting chemical elements:

Manganese

(Mn)

2 atoms

The binding sites of Chlorine atom in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State (pdb code 8e6i). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State, PDB code: 8e6i:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl503 b:63.0 occ:1.00 O A:HOH646 3.4 41.2 1.0 CB A:ALA203 3.9 46.3 1.0 NE2 A:HIS287 4.5 48.0 1.0 CD2 A:HIS287 4.6 49.7 1.0

Chlorine binding site 2 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter M230A Mutant in An Inward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl504 b:64.2 occ:1.00 O A:HOH609 2.5 56.6 1.0 O A:HOH664 2.6 44.1 1.0 NE A:ARG110 3.5 48.3 1.0 NH2 A:ARG110 3.6 41.5 1.0 NE A:ARG398 3.7 55.5 1.0 CD A:ARG398 3.7 50.4 1.0 CZ A:ARG398 3.8 55.3 1.0 O A:HOH651 3.8 49.9 1.0 CE1 A:PHE395 3.8 45.7 1.0 NH1 A:ARG398 3.8 49.4 1.0 NE1 A:TRP111 4.0 54.8 1.0 CZ A:ARG110 4.0 41.0 1.0 CD1 A:PHE395 4.3 42.5 1.0 O A:HOH624 4.3 41.1 1.0 NH2 A:ARG398 4.4 45.6 1.0 CD1 A:TRP111 4.5 49.9 1.0 CD A:ARG110 4.6 43.0 1.0 C24 A:OLC527 4.6 66.0 1.0 CB A:ARG110 4.7 42.1 1.0 O23 A:OLC527 4.7 66.0 1.0 CZ A:PHE395 4.9 43.4 1.0

S.Ray, S.P.Berry, E.A.Wilson, C.H.Zhang, M.Shekhar, A.Singharoy, R.Gaudet. High-Resolution Structures with Bound Mn 2+ and Cd 2+ Map the Metal Import Pathway in An Nramp Transporter. Elife V. 12 2023.
ISSN: ESSN 2050-084X
PubMed: 37039477
DOI: 10.7554/ELIFE.84006