Chlorine in PDB 8e6l: X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State

The structure of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State, PDB code: 8e6l was solved by S.Ray, R.Gaudet, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.32 / 3.12
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	58.514, 71.64, 98.895, 90, 90, 90
R / Rfree (%)	21.9 / 27.3

The structure of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Chlorine atom in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State (pdb code 8e6l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State, PDB code: 8e6l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl502 b:62.2 occ:1.00 NE2 A:HIS287 3.6 56.4 1.0 N A:TYR72 3.8 46.5 1.0 CD2 A:HIS287 3.8 56.2 1.0 CD2 A:TYR72 3.9 51.0 1.0 CB A:ALA203 3.9 55.7 1.0 CA A:GLY71 3.9 52.1 1.0 C A:GLY71 4.4 49.3 1.0 CE2 A:TYR72 4.5 50.7 1.0 O A:TYR70 4.6 56.5 1.0 CE1 A:HIS287 4.7 58.9 1.0 CG A:TYR72 4.7 50.4 1.0 CA A:TYR72 4.7 47.3 1.0 N A:SER73 4.8 52.3 1.0 CB A:TYR72 4.8 45.8 1.0 N A:GLY71 4.9 57.0 1.0 CG A:HIS287 5.0 54.9 1.0

Chlorine binding site 2 out of 2 in the X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter D296A Mutant in An Inward-Open, Manganese-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl503 b:66.2 occ:1.00 O23 A:OLC504 3.7 73.4 1.0 NE A:ARG110 3.7 51.0 1.0 NE1 A:TRP111 3.8 48.1 1.0 NH2 A:ARG110 4.0 57.4 1.0 CZ A:ARG398 4.0 59.9 1.0 NE A:ARG398 4.0 59.0 1.0 C21 A:OLC504 4.0 63.7 1.0 NH1 A:ARG398 4.0 64.6 1.0 CD A:ARG398 4.1 55.8 1.0 CE1 A:PHE395 4.2 52.1 1.0 CD1 A:TRP111 4.2 44.7 1.0 CZ A:ARG110 4.3 53.3 1.0 CB A:ARG110 4.5 51.0 1.0 C22 A:OLC504 4.5 63.1 1.0 NH2 A:ARG398 4.6 59.5 1.0 CD A:ARG110 4.7 49.5 1.0 O20 A:OLC504 4.8 65.7 1.0 O25 A:OLC504 4.8 63.8 1.0 CD1 A:PHE395 4.8 53.7 1.0 CE2 A:TRP111 5.0 51.1 1.0

S.Ray, S.P.Berry, E.A.Wilson, C.H.Zhang, M.Shekhar, A.Singharoy, R.Gaudet. High-Resolution Structures with Bound Mn 2+ and Cd 2+ Map the Metal Import Pathway in An Nramp Transporter. Elife V. 12 2023.
ISSN: ESSN 2050-084X
PubMed: 37039477
DOI: 10.7554/ELIFE.84006