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Chlorine in PDB 8fol: The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form

Enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form

All present enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form:
1.97.1.4;

Protein crystallography data

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form, PDB code: 8fol was solved by J.D.Moody, A.J.Saxton, A.Galambas, C.M.Lawrence, J.B.Broderick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.37 / 2.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 49.653, 59.011, 96.792, 90, 90, 90
R / Rfree (%) 19.5 / 21.4

Other elements in 8fol:

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form also contains other interesting chemical elements:

Potassium (K) 1 atom
Iron (Fe) 4 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form (pdb code 8fol). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form, PDB code: 8fol:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 8fol

Go back to Chlorine Binding Sites List in 8fol
Chlorine binding site 1 out of 3 in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl304

b:87.0
occ:1.00
 NE2 A:HIS111 3.3 107.9 1.0
CD2 A:HIS111 3.9 99.7 1.0
CE1 A:HIS111 4.1 102.9 1.0
CG A:HIS111 4.9 99.5 1.0
ND1 A:HIS111 5.0 101.6 1.0

Chlorine binding site 2 out of 3 in 8fol

Go back to Chlorine Binding Sites List in 8fol
Chlorine binding site 2 out of 3 in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl305

b:82.4
occ:1.00
 NH2 A:ARG151 3.3 69.1 1.0
NE A:ARG151 3.6 79.1 1.0
CD1 A:TYR112 3.6 67.1 1.0
CZ A:ARG151 3.7 65.8 1.0
CE1 A:TYR112 3.8 67.0 1.0
CG A:TYR112 3.8 68.6 1.0
CZ A:TYR112 4.1 63.3 1.0
CD2 A:TYR112 4.1 57.7 1.0
CE2 A:TYR112 4.2 63.5 1.0
CB A:TYR112 4.5 71.7 1.0
CG A:ARG151 4.5 56.8 1.0
CD A:ARG151 4.6 61.1 1.0
CD1 A:TYR155 4.6 51.6 1.0
NH1 A:ARG151 4.7 60.7 1.0
OH A:TYR112 4.8 50.5 1.0
CE1 A:TYR155 5.0 53.8 1.0

Chlorine binding site 3 out of 3 in 8fol

Go back to Chlorine Binding Sites List in 8fol
Chlorine binding site 3 out of 3 in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl306

b:68.3
occ:1.00
 OD1 A:ASN38 3.8 61.4 1.0
CE1 A:HIS7 3.8 52.2 1.0
ND1 A:HIS7 3.9 51.8 1.0
CG A:ASN38 4.2 56.3 1.0
OG A:SER8 4.3 42.5 1.0
NZ A:LYS208 4.3 66.1 1.0
CG A:PHE25 4.4 42.7 1.0
NE2 A:HIS7 4.5 51.4 1.0
ND2 A:ASN38 4.5 54.1 1.0
CD2 A:PHE25 4.5 41.2 1.0
CD1 A:PHE25 4.5 41.3 1.0
CG A:HIS7 4.7 45.8 1.0
CE2 A:PHE25 4.7 37.2 1.0
CB A:PHE25 4.8 43.6 1.0
CE1 A:PHE25 4.8 41.7 1.0
CZ A:PHE25 4.9 42.7 1.0
CB A:ASN38 4.9 55.7 1.0

Reference:

J.D.Moody, S.Hill, M.N.Lundahl, A.J.Saxton, A.Galambas, W.E.Broderick, C.M.Lawrence, J.B.Broderick. Computational Engineering of Previously Crystallized Pyruvate Formate-Lyase Activating Enzyme Reveals Insights Into Sam Binding and Reductive Cleavage. J.Biol.Chem. 04791 2023.
ISSN: ESSN 1083-351X
PubMed: 37156396
DOI: 10.1016/J.JBC.2023.104791
Page generated: Sun Jul 13 11:32:05 2025

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