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Chlorine in PDB 8rjm: Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).

Enzymatic activity of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).

All present enzymatic activity of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).:
2.7.13.3;

Protein crystallography data

The structure of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A)., PDB code: 8rjm was solved by L.Sauthof, A.Schmidt, M.Szczepek, A.S.Brewster, J.F.Kern, P.Scheerer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.75 / 2.15
Space group P 63 2 2
Cell size a, b, c (Å), α, β, γ (°) 184.98, 184.98, 182.538, 90, 90, 120
R / Rfree (%) 20.4 / 21.7

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). (pdb code 8rjm). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A)., PDB code: 8rjm:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in 8rjm

Go back to Chlorine Binding Sites List in 8rjm
Chlorine binding site 1 out of 5 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl603

b:93.9
occ:1.00
N A:HIS373 3.8 68.1 1.0
CB A:HIS373 4.1 74.8 1.0
CA A:PRO372 4.4 64.2 1.0
CA A:HIS373 4.5 71.3 1.0
C A:PRO372 4.6 66.1 1.0

Chlorine binding site 2 out of 5 in 8rjm

Go back to Chlorine Binding Sites List in 8rjm
Chlorine binding site 2 out of 5 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl604

b:93.1
occ:1.00
NH1 A:ARG349 3.2 82.7 1.0
O A:HOH733 3.7 62.5 1.0
CD A:ARG349 3.7 75.4 1.0
CZ A:ARG349 4.2 80.9 1.0
NE A:ARG349 4.4 78.4 1.0
CG A:ARG349 4.5 70.0 1.0

Chlorine binding site 3 out of 5 in 8rjm

Go back to Chlorine Binding Sites List in 8rjm
Chlorine binding site 3 out of 5 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl605

b:95.5
occ:1.00
O A:HOH770 2.9 65.5 1.0
NZ A:LYS174 3.6 50.8 1.0
NH1 A:ARG166 3.8 52.8 1.0
NH2 A:ARG166 3.9 53.4 1.0
CZ A:ARG166 4.3 51.9 1.0
CE A:LYS174 4.8 48.8 1.0

Chlorine binding site 4 out of 5 in 8rjm

Go back to Chlorine Binding Sites List in 8rjm
Chlorine binding site 4 out of 5 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl604

b:105.0
occ:1.00
N B:HIS373 3.9 73.3 1.0
CB B:HIS373 4.2 78.4 1.0
CA B:HIS373 4.6 75.9 1.0
CA B:PRO372 4.6 70.3 1.0
C B:PRO372 4.8 71.5 1.0
CD2 B:HIS373 4.9 83.3 1.0
CG B:HIS373 4.9 81.3 1.0

Chlorine binding site 5 out of 5 in 8rjm

Go back to Chlorine Binding Sites List in 8rjm
Chlorine binding site 5 out of 5 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A).


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in Its Pfr State (I0A). within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl605

b:97.5
occ:1.00
O B:HOH829 2.8 67.3 1.0
O B:HOH823 2.9 70.5 1.0
NZ B:LYS174 3.1 56.3 1.0
NH2 B:ARG166 3.7 61.0 1.0
NH1 B:ARG166 3.7 61.0 1.0
CE B:LYS174 4.0 54.8 1.0
CZ B:ARG166 4.1 60.2 1.0
CD B:LYS174 4.5 52.1 1.0
O B:HOH816 5.0 72.6 1.0
O B:HOH821 5.0 71.8 1.0

Reference:

L.Sauthof, M.Szczepek, A.Schmidt, A.Bhowmick, M.Dasgupta, M.J.Mackintosh, S.Gul, F.D.Fuller, R.Chatterjee, I.D.Young, N.Michael, N.A.Heyder, B.Bauer, A.Koch, I.Bogacz, I.-S.Kim, P.S.Simon, A.Butryn, P.Aller, V.U.Chukhutsina, J.M.Baxter, C.D.M.Hutchison, D.Liebschner, B.Poon, N.K.Sauter, M.D.Miller, G.N.Phillips Jr., R.Alonso-Mori, M.S.Hunter, A.Batyuk, S.Owada, K.Tono, R.Tanaka, J.J.Van Thor, N.Krauss, T.Lamparter, A.S.Brewster, I.Schapiro, A.M.Orville, V.K.Yachandra, J.Yano, P.Hildebrandt, J.F.Kern, P.Scheerer. Serial-Femtosecond Crystallography Reveals How A Phytochrome Variant Couples Chromophore and Protein Structural Changes Sci Adv 2025.
ISSN: ESSN 2375-2548
Page generated: Sun Jul 13 13:56:47 2025

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