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Chlorine in PDB 8rjr: Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State.

Enzymatic activity of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State.

All present enzymatic activity of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State.:
2.7.13.3;

Protein crystallography data

The structure of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State., PDB code: 8rjr was solved by L.Sauthof, A.Schmidt, M.Szczepek, A.S.Brewster, J.F.Kern, P.Scheerer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 90.32 / 2.30
Space group P 63 2 2
Cell size a, b, c (Å), α, β, γ (°) 183.756, 183.756, 180.318, 90, 90, 120
R / Rfree (%) 24.9 / 28.5

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State. (pdb code 8rjr). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State., PDB code: 8rjr:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 8rjr

Go back to Chlorine Binding Sites List in 8rjr
Chlorine binding site 1 out of 2 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl603

b:82.1
occ:1.00
O A:HOH748 2.9 53.3 1.0
O A:HOH777 3.4 57.1 1.0
OD1 A:ASP324 3.4 72.5 1.0
O A:HIS320 4.1 46.4 1.0
CB A:LEU323 4.3 43.0 1.0
CG A:ASP324 4.6 65.8 1.0
ND1 A:HIS320 4.6 46.2 1.0
CD1 A:LEU327 4.6 49.1 1.0
CA A:HIS320 4.7 43.7 1.0
CG2 A:THR487 4.7 42.6 1.0
CG A:HIS320 4.8 43.8 1.0
CD1 A:LEU323 4.8 41.0 1.0
N A:ASP324 4.9 50.3 1.0
CB A:HIS320 4.9 43.9 1.0
C A:HIS320 4.9 43.8 1.0

Chlorine binding site 2 out of 2 in 8rjr

Go back to Chlorine Binding Sites List in 8rjr
Chlorine binding site 2 out of 2 in the Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Serial Femtosecond X-Ray Structure of A Fluorescence Optimized Bathy Phytochrome PAIRFP2 Derived From Wild-Type AGP2 in I4 Intermediate State. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl604

b:98.2
occ:1.00
O A:HOH746 3.2 60.8 1.0
NZ A:LYS109 3.2 76.1 1.0
CE A:LYS109 3.5 73.4 1.0
NE2 B:HIS7 4.2 114.5 1.0
CD A:LYS109 4.7 70.2 1.0
CD2 B:HIS7 4.8 112.3 1.0
CE1 B:HIS7 4.9 114.1 1.0

Reference:

L.Sauthof, M.Szczepek, A.Schmidt, A.Bhowmick, M.Dasgupta, M.J.Mackintosh, S.Gul, F.D.Fuller, R.Chatterjee, I.D.Young, N.Michael, N.A.Heyder, B.Bauer, A.Koch, I.Bogacz, I.-S.Kim, P.S.Simon, A.Butryn, P.Aller, V.U.Chukhutsina, J.M.Baxter, C.D.M.Hutchison, D.Liebschner, B.Poon, N.K.Sauter, M.D.Miller, G.N.Phillips Jr., R.Alonso-Mori, M.S.Hunter, A.Batyuk, S.Owada, K.Tono, R.Tanaka, J.J.Van Thor, N.Krauss, T.Lamparter, A.S.Brewster, I.Schapiro, A.M.Orville, V.K.Yachandra, J.Yano, P.Hildebrandt, J.F.Kern, P.Scheerer. Serial-Femtosecond Crystallography Reveals How A Phytochrome Variant Couples Chromophore and Protein Structural Changes Sci Adv 2025.
ISSN: ESSN 2375-2548
Page generated: Sun Jul 13 13:57:04 2025

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