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Chlorine in PDB 8xi1: Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines

Protein crystallography data

The structure of Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines, PDB code: 8xi1 was solved by T.Kuga, N.Sunagawa, K.Igarashi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.76 / 1.37
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 83.441, 89.048, 88.989, 98.88, 110.55, 110.67
R / Rfree (%) 17.4 / 20

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines (pdb code 8xi1). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines, PDB code: 8xi1:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in 8xi1

Go back to Chlorine Binding Sites List in 8xi1
Chlorine binding site 1 out of 4 in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1003

b:12.2
occ:1.00
H A:LEU887 2.5 14.2 1.0
H A:THR491 2.5 11.7 1.0
H A:GLN490 2.7 14.4 1.0
HA3 A:GLY489 2.9 14.8 1.0
HD2 A:HIS140 3.1 13.1 1.0
N A:GLN490 3.1 12.0 1.0
HA A:PRO886 3.2 11.7 1.0
HD11 A:LEU887 3.2 20.1 1.0
O A:HOH1656 3.2 19.0 1.0
H A:GLN492 3.3 16.3 1.0
N A:LEU887 3.3 11.9 1.0
HB3 A:GLN490 3.3 12.7 1.0
HE2 A:HIS140 3.3 13.7 0.0
HB2 A:LEU887 3.3 15.9 1.0
HG A:LEU887 3.4 14.6 1.0
N A:THR491 3.4 9.7 1.0
OG1 A:THR491 3.5 11.0 1.0
CB A:PRO886 3.6 11.8 1.0
CD2 A:HIS140 3.7 10.9 1.0
CA A:GLY489 3.7 12.3 1.0
CA A:PRO886 3.7 9.7 1.0
C A:GLY489 3.7 13.3 1.0
NE2 A:HIS140 3.8 11.4 1.0
CG A:LEU887 3.9 12.2 1.0
CA A:GLN490 3.9 10.8 1.0
CD1 A:LEU887 3.9 16.7 1.0
CB A:LEU887 3.9 13.3 1.0
HG1 A:THR491 4.0 13.2 0.0
C A:PRO886 4.0 14.2 1.0
N A:GLN492 4.0 13.5 1.0
CB A:GLN490 4.0 10.6 1.0
C A:GLN490 4.1 10.4 1.0
HA2 A:GLY489 4.2 14.8 1.0
HB3 A:GLN492 4.2 21.5 1.0
CA A:LEU887 4.2 14.0 1.0
HD12 A:LEU887 4.3 20.1 1.0
CA A:THR491 4.3 11.9 1.0
HB2 A:GLN490 4.4 12.7 1.0
H A:LEU888 4.4 18.1 1.0
HB2 A:GLN492 4.5 21.5 1.0
CB A:THR491 4.5 10.4 1.0
C A:THR491 4.6 15.8 1.0
HD13 A:LEU887 4.7 20.1 1.0
N A:GLY489 4.7 12.2 1.0
CB A:GLN492 4.7 17.9 1.0
O A:GLY489 4.8 16.8 1.0
HG23 A:THR491 4.8 15.0 1.0
HB3 A:LEU887 4.8 15.9 1.0
HA A:GLN490 4.8 13.0 1.0
HA A:LEU887 4.9 16.8 1.0
O A:PHE488 5.0 19.1 1.0
CG A:HIS140 5.0 11.4 1.0

Chlorine binding site 2 out of 4 in 8xi1

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Chlorine binding site 2 out of 4 in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1004

b:13.6
occ:1.00
HD21 A:ASN623 2.4 19.2 1.0
HE A:ARG717 2.4 15.4 1.0
HD22 A:ASN751 2.5 15.6 1.0
HH21 A:ARG717 2.6 16.1 1.0
HB2 A:ASN623 2.8 17.8 1.0
HB2 A:ASN751 2.9 14.2 1.0
HD2 A:PHE749 3.1 16.2 1.0
O A:HOH1559 3.1 14.4 1.0
ND2 A:ASN623 3.2 16.0 1.0
NE A:ARG717 3.2 12.8 1.0
HZ3 A:TRP622 3.3 16.4 1.0
HE3 A:TRP622 3.3 14.9 1.0
ND2 A:ASN751 3.3 13.0 1.0
NH2 A:ARG717 3.4 13.4 1.0
HD12 A:LEU786 3.7 18.8 1.0
CB A:ASN623 3.7 14.8 1.0
HD22 A:ASN623 3.7 19.2 1.0
CZ A:ARG717 3.8 13.4 1.0
CB A:ASN751 3.8 11.8 1.0
CZ3 A:TRP622 3.8 13.7 1.0
CE3 A:TRP622 3.8 12.4 1.0
HD21 A:ASN751 3.9 15.6 1.0
CD2 A:PHE749 3.9 13.5 1.0
HB3 A:PHE749 3.9 11.9 1.0
CG A:ASN623 3.9 17.2 1.0
HB3 A:ASN623 4.0 17.8 1.0
HB3 A:ASN751 4.1 14.2 1.0
CG A:ASN751 4.1 12.6 1.0
HH22 A:ARG717 4.1 16.1 1.0
H A:ASN751 4.2 14.5 1.0
HD3 A:ARG717 4.2 15.9 1.0
CD A:ARG717 4.3 13.3 1.0
HD12 A:LEU626 4.4 17.9 1.0
HD22 A:LEU786 4.4 21.2 1.0
HG A:LEU786 4.4 17.2 1.0
HE2 A:PHE749 4.4 16.9 1.0
CD1 A:LEU786 4.5 15.7 1.0
HD11 A:LEU786 4.6 18.8 1.0
SD A:MET666 4.6 12.8 1.0
CE2 A:PHE749 4.6 14.1 1.0
HG2 A:ARG717 4.7 16.3 1.0
CB A:PHE749 4.7 9.9 1.0
CG A:PHE749 4.8 11.4 1.0
CA A:ASN623 4.8 14.2 1.0
CG A:LEU786 4.9 14.3 1.0
N A:ASN751 4.9 12.1 1.0
H A:ASN623 4.9 15.6 1.0
HA A:ASN623 4.9 17.1 1.0
CA A:ASN751 4.9 12.7 1.0
CH2 A:TRP622 5.0 14.2 1.0

Chlorine binding site 3 out of 4 in 8xi1

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Chlorine binding site 3 out of 4 in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl1005

b:13.4
occ:1.00
HE B:ARG717 2.4 16.7 1.0
HD21 B:ASN623 2.4 19.6 1.0
HH21 B:ARG717 2.5 17.7 1.0
HD21 B:ASN751 2.5 17.2 1.0
HB2 B:ASN623 2.8 17.2 1.0
HB2 B:ASN751 2.9 14.5 1.0
HD2 B:PHE749 3.1 16.7 1.0
O B:HOH1493 3.2 12.7 1.0
NE B:ARG717 3.2 13.9 1.0
ND2 B:ASN623 3.2 16.3 1.0
HZ3 B:TRP622 3.2 15.8 1.0
NH2 B:ARG717 3.3 14.7 1.0
HE3 B:TRP622 3.3 14.8 1.0
ND2 B:ASN751 3.4 14.4 1.0
CZ B:ARG717 3.7 12.5 1.0
CB B:ASN623 3.7 14.4 1.0
HD12 B:LEU786 3.7 19.4 1.0
CB B:ASN751 3.7 12.1 1.0
HD22 B:ASN623 3.8 19.6 1.0
CZ3 B:TRP622 3.8 13.2 1.0
CE3 B:TRP622 3.8 12.4 1.0
HB3 B:PHE749 3.9 14.1 1.0
CD2 B:PHE749 3.9 13.9 1.0
HD22 B:ASN751 3.9 17.2 1.0
CG B:ASN623 3.9 19.5 1.0
HB3 B:ASN751 4.0 14.5 1.0
HH22 B:ARG717 4.0 17.7 1.0
HB3 B:ASN623 4.0 17.2 1.0
CG B:ASN751 4.1 11.9 1.0
H B:ASN751 4.2 13.2 1.0
HD3 B:ARG717 4.2 16.2 1.0
HG B:LEU786 4.3 17.3 1.0
CD B:ARG717 4.3 13.5 1.0
HE2 B:PHE749 4.4 16.2 1.0
HD22 B:LEU786 4.5 22.3 1.0
HD12 B:LEU626 4.5 17.9 1.0
SD B:MET666 4.5 12.7 1.0
CD1 B:LEU786 4.6 16.1 1.0
CE2 B:PHE749 4.6 13.5 1.0
HG2 B:ARG717 4.7 14.2 1.0
CB B:PHE749 4.7 11.8 1.0
CG B:PHE749 4.8 12.0 1.0
HD11 B:LEU786 4.8 19.4 1.0
CA B:ASN623 4.9 14.2 1.0
CG B:LEU786 4.9 14.4 1.0
N B:ASN751 4.9 11.0 1.0
CA B:ASN751 4.9 11.3 1.0
HA B:ASN623 4.9 17.1 1.0
HD11 B:LEU626 5.0 17.9 1.0
H B:ASN623 5.0 16.6 1.0
CH2 B:TRP622 5.0 12.0 1.0

Chlorine binding site 4 out of 4 in 8xi1

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Chlorine binding site 4 out of 4 in the Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Cellodextrin Phosphorylase From Clostridium Thermocellum Mutant - All Cysteine Residues Were Substituted with Serines within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl1006

b:11.9
occ:1.00
H B:LEU887 2.5 13.0 1.0
H B:THR491 2.5 14.3 1.0
H B:GLN490 2.7 13.2 1.0
HA3 B:GLY489 2.9 15.2 1.0
HD2 B:HIS140 3.1 13.8 1.0
N B:GLN490 3.1 11.0 1.0
HA B:PRO886 3.1 12.8 1.0
HD11 B:LEU887 3.2 18.8 1.0
O B:HOH1688 3.2 18.2 1.0
H B:GLN492 3.3 15.0 1.0
N B:LEU887 3.3 10.8 1.0
HB3 B:GLN490 3.3 14.4 1.0
HB2 B:PRO886 3.3 16.5 1.0
HE2 B:HIS140 3.3 13.8 0.0
N B:THR491 3.4 11.9 1.0
HG B:LEU887 3.4 14.8 1.0
HB3 B:PRO886 3.4 16.5 1.0
HB2 B:LEU887 3.4 15.0 1.0
OG1 B:THR491 3.5 12.0 1.0
CB B:PRO886 3.6 13.7 1.0
CA B:GLY489 3.7 12.6 1.0
CD2 B:HIS140 3.7 11.4 1.0
CA B:PRO886 3.7 10.7 1.0
C B:GLY489 3.7 12.0 1.0
NE2 B:HIS140 3.8 11.5 1.0
CA B:GLN490 3.9 11.8 1.0
CG B:LEU887 3.9 12.3 1.0
CD1 B:LEU887 3.9 15.6 1.0
N B:GLN492 4.0 12.5 1.0
CB B:LEU887 4.0 12.5 1.0
CB B:GLN490 4.0 12.0 1.0
C B:PRO886 4.0 12.9 1.0
HG1 B:THR491 4.0 14.4 0.0
C B:GLN490 4.1 12.9 1.0
HA2 B:GLY489 4.1 15.2 1.0
HB3 B:GLN492 4.2 20.5 1.0
CA B:LEU887 4.2 12.1 1.0
CA B:THR491 4.3 12.1 1.0
HB2 B:GLN490 4.3 14.4 1.0
HD12 B:LEU887 4.4 18.8 1.0
HB2 B:GLN492 4.4 20.5 1.0
H B:LEU888 4.4 17.9 1.0
CB B:THR491 4.5 11.4 1.0
C B:THR491 4.5 15.0 1.0
HD13 B:LEU887 4.7 18.8 1.0
CB B:GLN492 4.7 17.1 1.0
N B:GLY489 4.7 12.2 1.0
O B:GLY489 4.7 15.7 1.0
HG23 B:THR491 4.8 15.6 1.0
HA B:GLN490 4.8 14.2 1.0
HB3 B:LEU887 4.9 15.0 1.0
HA B:LEU887 4.9 14.5 1.0
CA B:GLN492 5.0 14.4 1.0
O B:PHE488 5.0 16.5 0.9

Reference:

T.Kuga, N.Sunagawa, K.Igarashi. Effect of Free Cysteine Residues to Serine Mutation on Cellodextrin Phosphorylase To Be Published.
Page generated: Sat Feb 8 17:23:54 2025

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