Chlorine in PDB 1ky0: Methionine Core Mutant of T4 Lysozyme

All present enzymatic activity of Methionine Core Mutant of T4 Lysozyme:
3.2.1.17;

The structure of Methionine Core Mutant of T4 Lysozyme, PDB code: 1ky0 was solved by N.C.Gassner, W.A.Baase, B.H.Mooers, R.D.Busam, L.H.Weaver, J.D.Lindstrom, M.L.Quillin, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.97
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.300, 61.300, 96.500, 90.00, 90.00, 120.00
R / Rfree (%)	16 / n/a

The binding sites of Chlorine atom in the Methionine Core Mutant of T4 Lysozyme (pdb code 1ky0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Methionine Core Mutant of T4 Lysozyme, PDB code: 1ky0:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Methionine Core Mutant of T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Methionine Core Mutant of T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:50.1 occ:1.00 O A:HOH209 2.9 36.8 1.0 N A:ARG145 3.1 9.7 1.0 CB A:ARG145 3.5 11.0 1.0 N A:ASN144 3.6 7.1 1.0 C A:THR142 3.6 17.4 1.0 CB A:THR142 3.6 13.4 1.0 CB A:ASN144 3.7 17.3 1.0 CA A:THR142 3.7 8.6 1.0 O A:THR142 3.8 9.3 1.0 CA A:ASN144 3.9 11.7 1.0 CA A:ARG145 3.9 11.1 1.0 C A:ASN144 3.9 24.5 1.0 N A:PRO143 4.2 14.1 1.0 O A:HOH230 4.3 33.1 1.0 CG2 A:THR142 4.4 16.0 1.0 C A:PRO143 4.4 20.1 1.0 O A:HOH245 4.5 42.8 1.0 CG A:ASN144 4.8 25.8 1.0 CD A:PRO143 4.8 13.6 1.0 OG1 A:THR142 4.8 16.0 1.0 O A:HOH237 4.9 29.4 1.0 CA A:PRO143 4.9 12.9 1.0 CG A:ARG145 4.9 15.6 1.0

Chlorine binding site 2 out of 2 in the Methionine Core Mutant of T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Methionine Core Mutant of T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:35.2 occ:0.50 O A:HOH215 3.0 20.3 1.0 O A:HOH200 3.2 33.8 1.0 O A:HOH182 3.4 23.4 1.0 O A:HOH246 3.6 49.9 1.0 CB A:ALA49 4.0 19.2 1.0 CE1 A:HIS31 4.1 18.9 1.0 NE2 A:HIS31 4.2 16.0 1.0 O A:HOH282 4.4 36.0 1.0 NE2 A:GLN69 4.5 13.2 1.0 CA A:ALA49 4.5 23.3 1.0 O A:HOH202 4.7 58.1 1.0 CD2 A:LEU66 4.8 17.8 1.0

N.C.Gassner, W.A.Baase, B.H.Mooers, R.D.Busam, L.H.Weaver, J.D.Lindstrom, M.L.Quillin, B.W.Matthews. Multiple Methionine Substitutions Are Tolerated in T4 Lysozyme and Have Coupled Effects on Folding and Stability Biophys.Chem. V. 100 325 2003.
ISSN: ISSN 0301-4622
PubMed: 12646375
DOI: 10.1016/S0301-4622(02)00290-9