Chlorine in PDB 3dxb: Structure of the Uhm Domain of PUF60 Fused to Thioredoxin

The structure of Structure of the Uhm Domain of PUF60 Fused to Thioredoxin, PDB code: 3dxb was solved by L.Corsini, M.Hothorn, K.Scheffzek, G.Stier, M.Sattler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.75 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.120, 89.430, 299.390, 90.00, 90.00, 90.00
R / Rfree (%)	21.1 / 27.1

The binding sites of Chlorine atom in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin (pdb code 3dxb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin, PDB code: 3dxb:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of the Uhm Domain of PUF60 Fused to Thioredoxin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1 b:37.3 occ:1.00 O A:HOH695 2.2 38.0 1.0 ND1 A:HIS350 3.5 18.0 1.0 O A:HOH658 3.5 43.5 1.0 CZ2 A:TRP372 3.6 13.8 1.0 NE1 A:TRP372 3.8 13.3 1.0 O A:HOH562 3.9 13.0 1.0 CE2 A:TRP372 4.0 13.3 1.0 CB A:HIS350 4.1 17.8 1.0 CG A:HIS350 4.3 18.0 1.0 O A:LYS401 4.4 16.9 1.0 CB A:LYS401 4.4 16.6 1.0 CE1 A:HIS350 4.5 17.8 1.0 CA A:HIS350 4.6 17.9 1.0 CH2 A:TRP372 4.7 13.8 1.0 C A:LYS401 4.8 16.8 1.0 CE A:LYS401 4.8 19.1 1.0 CB A:ASN403 4.8 17.8 1.0 N A:ASN403 4.9 17.7 1.0

Chlorine binding site 2 out of 4 in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of the Uhm Domain of PUF60 Fused to Thioredoxin within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl1 b:46.0 occ:1.00 NE1 E:TRP533 3.4 19.0 1.0 CE E:LYS538 4.0 19.9 1.0 NZ E:LYS538 4.0 20.0 1.0 CD1 E:TRP533 4.2 19.3 1.0 CE2 E:TRP533 4.5 18.9 1.0 CD E:LYS538 4.7 19.7 1.0 O E:GLY531 4.7 19.9 1.0 CZ2 E:TRP533 4.9 18.7 1.0

Chlorine binding site 3 out of 4 in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of the Uhm Domain of PUF60 Fused to Thioredoxin within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl1 b:45.5 occ:1.00 CG F:ARG532 4.1 16.3 1.0 N F:TRP533 4.6 14.0 1.0 CA F:ARG532 4.8 14.6 1.0 CD1 F:TRP533 4.8 12.2 1.0 CD F:ARG532 5.0 19.1 1.0 NE F:ARG532 5.0 21.0 1.0

Chlorine binding site 4 out of 4 in the Structure of the Uhm Domain of PUF60 Fused to Thioredoxin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of the Uhm Domain of PUF60 Fused to Thioredoxin within 5.0Å range: probe atom residue distance (Å) B Occ H:Cl1 b:42.6 occ:1.00 NH2 H:ARG548 3.6 19.3 1.0 NH1 H:ARG548 4.2 19.1 1.0 CZ H:ARG548 4.3 18.8 1.0

L.Corsini, M.Hothorn, G.Stier, V.Rybin, K.Scheffzek, T.J.Gibson, M.Sattler. Dimerization and Protein Binding Specificity of the U2AF Homology Motif of the Splicing Factor PUF60. J.Biol.Chem. V. 284 630 2009.
ISSN: ISSN 0021-9258
PubMed: 18974054
DOI: 10.1074/JBC.M805395200