Atomistry » Chlorine » PDB 3fr1-3fzn » 3fyp
Atomistry »
  Chlorine »
    PDB 3fr1-3fzn »
      3fyp »

Chlorine in PDB 3fyp: Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis

Enzymatic activity of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis

All present enzymatic activity of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis:
2.5.1.55;

Protein crystallography data

The structure of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis, PDB code: 3fyp was solved by G.B.Jameson, E.J.Parker, F.P.Cochrane, M.L.Patchett, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.96 / 1.85
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 81.781, 85.679, 163.828, 90.00, 90.00, 90.00
R / Rfree (%) 20.5 / 24.7

Other elements in 3fyp:

The structure of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis also contains other interesting chemical elements:

Manganese (Mn) 1 atom
Sodium (Na) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis (pdb code 3fyp). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis, PDB code: 3fyp:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 3fyp

Go back to Chlorine Binding Sites List in 3fyp
Chlorine binding site 1 out of 2 in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl281

b:33.2
occ:1.00
 CE1 A:HIS37 3.4 34.4 1.0
N A:GLU258 3.4 22.9 1.0
C A:HIS255 3.4 33.7 1.0
N A:LEU257 3.4 23.6 1.0
CA A:HIS255 3.5 35.8 1.0
CB A:LEU257 3.6 24.1 1.0
CB A:HIS255 3.6 31.9 1.0
N A:LEU256 3.7 30.9 1.0
NE2 A:HIS37 3.7 34.7 1.0
O A:HIS255 3.8 29.3 1.0
CA A:LEU257 3.9 23.2 1.0
CG A:GLU258 4.0 30.6 1.0
CG2 A:VAL41 4.0 22.4 1.0
CB A:GLU258 4.1 30.2 1.0
C A:LEU257 4.1 18.1 1.0
OE1 A:GLU258 4.3 30.2 1.0
CA A:GLU258 4.3 24.8 1.0
C A:LEU256 4.4 28.2 1.0
CD A:GLU258 4.5 31.9 1.0
ND1 A:HIS37 4.6 34.2 1.0
CG A:HIS255 4.7 31.5 1.0
CA A:LEU256 4.7 29.9 1.0
ND1 A:HIS255 4.7 34.6 1.0
CG A:LEU257 4.8 22.2 1.0
N A:HIS255 4.9 41.5 1.0

Chlorine binding site 2 out of 2 in 3fyp

Go back to Chlorine Binding Sites List in 3fyp
Chlorine binding site 2 out of 2 in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl282

b:37.8
occ:0.50
 O1 D:PEP281 0.6 23.5 0.5
C1 D:PEP281 1.5 24.5 0.5
O2' D:PEP281 2.3 26.0 0.5
C2 D:PEP281 2.7 23.5 0.5
O2 D:PEP281 2.9 21.1 0.5
NE2 D:GLN110 3.0 21.1 1.0
NZ D:LYS135 3.3 26.9 1.0
CE D:LYS52 3.4 37.2 1.0
CE1 D:HIS199 3.5 29.2 1.0
CZ D:PHE234 3.5 21.0 1.0
NZ D:LYS52 3.7 36.7 1.0
CE2 D:PHE234 3.8 20.8 1.0
CE D:LYS135 3.8 27.5 1.0
C3 D:PEP281 3.9 24.9 0.5
ND1 D:HIS199 4.1 23.7 1.0
CD D:GLN110 4.1 23.8 1.0
OE2 D:GLU236 4.2 40.7 1.0
O D:HOH485 4.2 23.8 1.0
NE2 D:HIS199 4.3 28.6 1.0
OE1 D:GLN110 4.4 22.1 1.0
P D:PEP281 4.5 22.3 0.5
CD D:LYS52 4.6 34.5 1.0
OD2 D:ASP196 4.7 22.9 1.0
CE1 D:PHE234 4.7 20.2 1.0
O D:HOH541 4.8 19.9 0.5
O1P D:PEP281 5.0 17.7 0.5

Reference:

F.C.Cochrane, T.V.Cookson, G.B.Jameson, E.J.Parker. Reversing Evolution: Re-Establishing Obligate Metal Ion Dependence in A Metal-Independent KDO8P Synthase J.Mol.Biol. V. 390 646 2009.
ISSN: ISSN 0022-2836
PubMed: 19447118
DOI: 10.1016/J.JMB.2009.05.014
Page generated: Fri Jul 11 05:18:58 2025

Last articles

Cl in 3ZOJ
Cl in 3ZOQ
Cl in 3ZNU
Cl in 3ZOA
Cl in 3ZO9
Cl in 3ZNX
Cl in 3ZNV
Cl in 3ZN5
Cl in 3ZNO
Cl in 3ZN6
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy