Chlorine in PDB 4ekv: Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding

The structure of Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding, PDB code: 4ekv was solved by I.H.Barrette-Ng, C.Honetschlaeger, S.L.Wong, K.K.S.Ng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.39 / 2.00
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	57.066, 57.066, 70.186, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 22.5

The binding sites of Chlorine atom in the Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding (pdb code 4ekv). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding, PDB code: 4ekv:

Chlorine binding site 1 out of 1 in the Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Streptavidin 8-Aa-Loop H127C Mutein with Reversible Biotin Binding within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:55.2 occ:1.00 O A:HOH368 2.8 52.4 1.0 N A:ASP36 2.9 41.5 1.0 CA A:ASP36 3.3 45.7 1.0 N A:ALA38 3.3 30.8 1.0 N A:GLY37 3.3 38.2 1.0 CB A:ASP36 3.3 48.1 1.0 C A:ASP36 3.4 42.3 1.0 C A:GLY34 3.5 41.1 1.0 CB A:ALA38 3.5 32.4 1.0 N A:ALA35 3.6 43.2 1.0 CA A:GLY34 3.7 38.5 1.0 O A:GLY34 3.9 41.5 1.0 C A:ALA35 4.0 43.0 1.0 CA A:ALA38 4.0 30.3 1.0 O A:ASP36 4.1 44.4 1.0 C A:GLY37 4.3 33.1 1.0 CA A:GLY37 4.3 32.8 1.0 CA A:ALA35 4.4 41.9 1.0 CG A:ASP36 4.4 59.9 1.0 N A:GLY34 4.7 36.1 1.0 NH1 A:ARG59 4.9 26.9 1.0 OD1 A:ASP36 4.9 66.2 1.0

V.J.O'sullivan, I.Barrette-Ng, E.Hommema, G.T.Hermanson, M.Schofield, S.C.Wu, C.Honetschlaeger, K.K.Ng, S.L.Wong. Development of A Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering A Mobile Loop As An Exit Door For Biotin. Plos One V. 7 35203 2012.
ISSN: ESSN 1932-6203
PubMed: 22536357
DOI: 10.1371/JOURNAL.PONE.0035203