Chlorine in PDB 4pc2: Elongation Factor Tu:Ts Complex with A Bound Gdp

The structure of Elongation Factor Tu:Ts Complex with A Bound Gdp, PDB code: 4pc2 was solved by S.S.Thirup, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.99 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	73.927, 108.510, 193.750, 90.00, 90.00, 90.00
R / Rfree (%)	16.5 / 21.4

The binding sites of Chlorine atom in the Elongation Factor Tu:Ts Complex with A Bound Gdp (pdb code 4pc2). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Elongation Factor Tu:Ts Complex with A Bound Gdp, PDB code: 4pc2:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Elongation Factor Tu:Ts Complex with A Bound Gdp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Elongation Factor Tu:Ts Complex with A Bound Gdp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl402 b:29.8 occ:0.85 H A:PHE323 2.5 26.4 1.0 HB2 A:PHE322 2.8 24.8 1.0 HA A:PHE322 3.2 25.5 1.0 HB2 A:TYR326 3.2 27.9 1.0 O A:HOH608 3.2 28.5 1.0 N A:PHE323 3.3 22.0 1.0 HD1 A:PHE323 3.3 27.8 1.0 HA A:TYR326 3.6 26.7 1.0 CB A:PHE322 3.6 20.6 1.0 HD1 A:TYR326 3.6 27.8 1.0 CA A:PHE322 3.7 21.2 1.0 HB2 A:PHE323 3.7 28.2 1.0 O A:HOH622 3.8 35.9 1.0 HB3 A:PHE322 3.8 24.8 1.0 CB A:TYR326 4.0 23.2 1.0 C A:PHE322 4.0 21.8 1.0 O A:PHE323 4.1 24.7 1.0 CA A:TYR326 4.1 22.2 1.0 H A:TYR326 4.1 27.1 1.0 N A:TYR326 4.1 22.6 1.0 CD1 A:PHE323 4.2 23.1 1.0 CA A:PHE323 4.2 22.8 1.0 CD1 A:TYR326 4.2 23.2 1.0 CB A:PHE323 4.4 23.5 1.0 C A:PHE323 4.4 25.4 1.0 CG A:TYR326 4.5 21.2 1.0 HH12 A:ARG318 4.6 27.1 1.0 HD1 A:PHE322 4.7 24.7 1.0 HB3 A:TYR326 4.8 27.9 1.0 C A:GLY325 4.8 22.7 1.0 CG A:PHE323 4.8 23.5 1.0 CG A:PHE322 4.8 20.4 1.0 H A:GLY325 4.8 33.9 1.0 HB2 B:ARG381 4.9 36.2 1.0 O C:HOH529 5.0 39.1 1.0

Chlorine binding site 2 out of 5 in the Elongation Factor Tu:Ts Complex with A Bound Gdp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Elongation Factor Tu:Ts Complex with A Bound Gdp within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl303 b:48.2 occ:1.00 H C:SER186 2.5 39.9 1.0 HE2 C:PHE179 2.7 33.8 1.0 HB3 C:SER186 2.8 44.9 1.0 HG C:SER186 2.9 44.4 1.0 HG21 C:VAL189 3.0 55.5 1.0 HZ C:PHE179 3.0 32.8 1.0 HG2 D:GLN203 3.1 37.0 1.0 N C:SER186 3.3 33.3 1.0 CE2 C:PHE179 3.4 28.2 1.0 OG C:SER186 3.4 37.0 1.0 CB C:SER186 3.5 37.4 1.0 HA C:VAL185 3.5 37.7 1.0 CZ C:PHE179 3.5 27.3 1.0 HG23 C:VAL189 3.6 55.5 1.0 CG2 C:VAL189 3.7 46.2 1.0 HB C:VAL189 3.8 50.7 1.0 HG13 C:VAL185 3.9 37.4 1.0 CA C:SER186 4.0 36.5 1.0 CG D:GLN203 4.1 30.8 1.0 CB C:VAL189 4.2 42.2 1.0 HG11 C:VAL189 4.3 50.1 1.0 C C:VAL185 4.3 32.7 1.0 HB2 C:SER186 4.3 44.9 1.0 CA C:VAL185 4.3 31.4 1.0 HG3 D:GLN203 4.5 37.0 1.0 O C:ASP184 4.5 31.6 1.0 HG22 C:VAL189 4.5 55.5 1.0 HB3 D:GLN203 4.5 40.0 1.0 HG12 C:VAL185 4.5 37.4 1.0 HA D:ILE200 4.5 33.4 1.0 CG1 C:VAL185 4.6 31.2 1.0 HA C:SER186 4.7 43.9 1.0 CD2 C:PHE179 4.7 26.7 1.0 HB3 D:ASP199 4.7 40.4 1.0 CB D:GLN203 4.8 33.4 1.0 CD D:GLN203 4.8 29.2 1.0 HG12 D:ILE200 4.8 32.9 1.0 CG1 C:VAL189 4.8 41.8 1.0 HB2 D:GLN203 4.8 40.0 1.0 CE1 C:PHE179 4.9 25.4 1.0 H C:VAL189 4.9 47.8 1.0 HD2 C:PHE179 5.0 32.1 1.0

Chlorine binding site 3 out of 5 in the Elongation Factor Tu:Ts Complex with A Bound Gdp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Elongation Factor Tu:Ts Complex with A Bound Gdp within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl402 b:34.8 occ:1.00 H B:PHE323 2.5 25.4 1.0 HB2 B:PHE322 2.9 24.0 1.0 O B:HOH626 3.0 45.0 1.0 HD1 B:PHE323 3.1 27.2 1.0 HH21 A:ARG381 3.2 61.9 1.0 HB2 B:TYR326 3.2 25.8 1.0 HA B:PHE322 3.2 24.8 1.0 O A:HOH584 3.2 28.8 1.0 N B:PHE323 3.3 21.2 1.0 O A:HOH599 3.4 48.9 1.0 CB B:PHE322 3.6 20.0 1.0 HD1 B:TYR326 3.6 27.2 1.0 HB2 B:PHE323 3.6 27.1 1.0 HA B:TYR326 3.7 24.7 1.0 CA B:PHE322 3.7 20.7 1.0 HB3 B:PHE322 3.9 24.0 1.0 NH2 A:ARG381 3.9 51.6 1.0 H B:TYR326 4.0 25.6 1.0 CB B:TYR326 4.0 21.5 1.0 HE A:ARG381 4.0 60.0 1.0 CD1 B:PHE323 4.0 22.7 1.0 C B:PHE322 4.0 20.7 1.0 O B:PHE323 4.1 22.8 1.0 CA B:TYR326 4.2 20.6 1.0 CA B:PHE323 4.2 21.8 1.0 N B:TYR326 4.2 21.3 1.0 CB B:PHE323 4.3 22.6 1.0 CD1 B:TYR326 4.3 22.7 1.0 HH22 A:ARG381 4.4 61.9 1.0 C B:PHE323 4.4 25.7 1.0 HH12 B:ARG318 4.6 26.4 1.0 CG B:TYR326 4.6 20.4 1.0 CG B:PHE323 4.6 22.8 1.0 NE A:ARG381 4.6 50.0 1.0 HD1 B:PHE322 4.7 23.1 1.0 CZ A:ARG381 4.7 51.1 1.0 HB3 B:TYR326 4.7 25.8 1.0 HB2 A:ARG381 4.8 40.5 1.0 CG B:PHE322 4.9 19.2 1.0 H B:GLY325 4.9 34.9 1.0 HE1 B:PHE323 5.0 27.7 1.0 C B:GLY325 5.0 22.2 1.0 CE1 B:PHE323 5.0 23.1 1.0

Chlorine binding site 4 out of 5 in the Elongation Factor Tu:Ts Complex with A Bound Gdp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Elongation Factor Tu:Ts Complex with A Bound Gdp within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl403 b:41.6 occ:0.91 HH22 B:ARG333 2.5 40.6 1.0 H B:ASP369 2.6 37.2 1.0 O B:HOH622 2.6 54.4 1.0 HB2 B:LEU372 2.8 31.0 1.0 HH12 B:ARG333 3.0 37.5 1.0 HA B:MET368 3.1 32.9 1.0 HD11 B:ILE298 3.1 37.0 1.0 N B:ASP369 3.2 31.0 1.0 HD22 B:LEU372 3.2 32.2 1.0 HH22 B:ARG233 3.2 58.0 1.0 HD13 B:LEU372 3.3 32.8 1.0 NH2 B:ARG333 3.3 33.8 1.0 HB2 B:ASP369 3.5 45.7 1.0 HB3 B:ASP369 3.6 45.7 1.0 HD12 B:ILE298 3.7 37.0 1.0 NH1 B:ARG333 3.7 31.2 1.0 CB B:LEU372 3.8 25.8 1.0 CD1 B:ILE298 3.8 30.8 1.0 C B:MET368 3.8 30.1 1.0 CA B:MET368 3.9 27.4 1.0 CB B:ASP369 3.9 38.1 1.0 HH21 B:ARG333 3.9 40.6 1.0 CZ B:ARG333 3.9 31.9 1.0 CA B:ASP369 4.0 33.2 1.0 O B:HOH526 4.0 45.6 1.0 NH2 B:ARG233 4.1 48.4 1.0 CD2 B:LEU372 4.1 26.8 1.0 CD1 B:LEU372 4.1 27.3 1.0 O B:ASP369 4.2 28.7 1.0 CG B:LEU372 4.2 26.7 1.0 HB3 B:LEU372 4.3 31.0 1.0 HH12 B:ARG233 4.3 59.5 1.0 HH21 B:ARG233 4.4 58.0 1.0 HH11 B:ARG333 4.4 37.5 1.0 HD13 B:ILE298 4.4 37.0 1.0 HA B:LEU372 4.5 32.2 1.0 C B:ASP369 4.5 30.5 1.0 HB2 B:MET368 4.5 33.6 1.0 HD23 B:LEU372 4.6 32.2 1.0 HD12 B:LEU372 4.6 32.8 1.0 HG13 B:ILE298 4.6 38.7 1.0 CA B:LEU372 4.6 26.8 1.0 H B:LEU372 4.6 31.4 1.0 HD21 B:LEU372 4.7 32.2 1.0 HD11 B:LEU372 4.7 32.8 1.0 N B:MET368 4.7 29.2 1.0 O B:HOH623 4.8 41.2 1.0 CB B:MET368 4.8 28.0 1.0 O B:MET368 4.8 29.2 1.0 O B:ALA367 4.8 28.6 1.0 CG1 B:ILE298 4.9 32.2 1.0 HA B:ASP369 4.9 39.9 1.0 N B:LEU372 4.9 26.2 1.0 NH1 B:ARG233 5.0 49.6 1.0 CZ B:ARG233 5.0 48.6 1.0

Chlorine binding site 5 out of 5 in the Elongation Factor Tu:Ts Complex with A Bound Gdp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Elongation Factor Tu:Ts Complex with A Bound Gdp within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl301 b:54.6 occ:1.00 HG D:SER186 2.4 51.5 1.0 H D:SER186 2.4 45.7 1.0 HG21 D:VAL189 2.8 44.1 1.0 HE2 D:PHE179 3.0 33.3 1.0 HB3 D:SER186 3.1 52.9 1.0 OG D:SER186 3.1 42.9 1.0 HZ D:PHE179 3.1 32.2 1.0 HG2 C:GLN203 3.2 38.2 1.0 N D:SER186 3.3 38.1 1.0 HG23 D:VAL189 3.4 44.1 1.0 HA D:VAL185 3.4 39.6 1.0 CG2 D:VAL189 3.5 36.7 1.0 CB D:SER186 3.5 44.1 1.0 CE2 D:PHE179 3.6 27.8 1.0 CZ D:PHE179 3.7 26.8 1.0 HB D:VAL189 3.8 41.5 1.0 HG13 D:VAL185 3.9 38.4 1.0 CA D:SER186 4.0 42.3 1.0 CB D:VAL189 4.2 34.6 1.0 CG C:GLN203 4.2 31.8 1.0 CA D:VAL185 4.2 33.0 1.0 C D:VAL185 4.2 36.5 1.0 HG22 D:VAL189 4.3 44.1 1.0 O D:ASP184 4.3 37.8 1.0 HG11 D:VAL189 4.3 39.5 1.0 HB2 D:SER186 4.4 52.9 1.0 HG12 D:VAL185 4.5 38.4 1.0 CG1 D:VAL185 4.6 32.0 1.0 HG3 C:GLN203 4.6 38.2 1.0 HB3 C:ASP199 4.6 41.5 1.0 HA C:ILE200 4.7 32.0 1.0 HB3 C:GLN203 4.7 42.5 1.0 HA D:SER186 4.7 50.8 1.0 CG1 D:VAL189 4.9 32.9 1.0 CD C:GLN203 4.9 30.1 1.0 H D:VAL189 4.9 43.8 1.0 CD2 D:PHE179 4.9 26.2 1.0 CB C:GLN203 5.0 35.4 1.0 HG12 C:ILE200 5.0 33.3 1.0

S.S.Thirup, L.B.Van, T.K.Nielsen, C.R.Knudsen. Structural Outline of the Detailed Mechanism For Elongation Factor Ts Mediated Guanine Nucleotide Exchange on Elongation Factor Tu To Be Published.