Chlorine in PDB 5i54: Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours

All present enzymatic activity of Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours:
3.2.1.17;

The structure of Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours, PDB code: 5i54 was solved by M.V.Hosur, T.Raskar, S.Khavnekar, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.95 / 1.61
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.040, 79.040, 36.600, 90.00, 90.00, 90.00
R / Rfree (%)	16.5 / 19.9

The binding sites of Chlorine atom in the Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours (pdb code 5i54). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours, PDB code: 5i54:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl208 b:17.9 occ:1.00 OH A:TYR23 2.9 11.9 1.0 O A:HOH370 3.3 17.7 1.0 O A:HOH401 3.4 29.9 1.0 CZ A:TYR23 3.6 12.1 1.0 CE2 A:TYR23 3.6 12.1 1.0 CA A:GLY104 4.1 13.6 1.0 O A:ARG21 4.6 14.9 1.0 N A:GLY104 4.8 14.2 1.0 CE1 A:TYR23 4.8 9.9 1.0 CD2 A:TYR23 4.9 10.9 1.0

Chlorine binding site 2 out of 3 in the Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl209 b:21.2 occ:1.00 O A:HOH397 2.9 31.1 1.0 OG A:SER24 3.1 17.1 1.0 N A:GLY26 3.1 12.6 1.0 CA A:GLY26 3.5 10.8 1.0 CB A:SER24 3.5 14.4 1.0 CA A:GLN121 3.6 17.0 1.0 CG A:GLN121 3.9 30.0 1.0 CD1 A:ILE124 3.9 22.4 1.0 N A:GLN121 3.9 16.8 1.0 CB A:GLN121 4.1 23.0 1.0 N A:LEU25 4.1 13.6 1.0 CG2 A:VAL120 4.3 15.4 1.0 C A:SER24 4.3 12.3 1.0 C A:LEU25 4.3 14.6 1.0 O A:VAL120 4.3 14.4 1.0 C A:VAL120 4.3 17.0 1.0 CG1 A:ILE124 4.4 19.1 1.0 CA A:SER24 4.5 14.0 1.0 C A:GLY26 4.6 11.2 1.0 CA A:LEU25 4.6 13.2 1.0 N A:ASN27 4.7 12.1 1.0 O A:SER24 4.8 12.6 1.0 C A:GLN121 4.8 16.6 1.0 CB A:LEU25 4.9 12.8 1.0 O A:GLN121 5.0 17.8 1.0

Chlorine binding site 3 out of 3 in the Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Exploring Onset of Lysozyme Denaturation By Urea - Soak Period 4 Hours within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl210 b:35.2 occ:1.00 NZ A:LYS33 3.5 24.9 1.0 CE2 A:PHE38 3.6 13.6 1.0 O A:HOH351 3.8 35.9 1.0 CZ3 A:TRP123 4.1 16.9 1.0 CE A:LYS33 4.1 21.0 1.0 CB A:ARG5 4.2 13.5 1.0 CD A:LYS33 4.2 17.6 1.0 CD A:ARG5 4.3 15.9 1.0 CZ A:PHE38 4.3 13.6 1.0 O A:HOH402 4.5 27.5 1.0 CD2 A:PHE38 4.5 15.1 1.0 CG A:ARG5 4.7 13.9 1.0 NE A:ARG5 4.8 17.5 1.0 CE3 A:TRP123 4.9 15.7 1.0 CH2 A:TRP123 5.0 18.2 1.0

T.Raskar, S.Khavnekar, M.Hosur. Time-Dependent X-Ray Diffraction Studies on Urea/Hen Egg White Lysozyme Complexes Reveal Structural Changes That Indicate Onset of Denaturation Sci Rep V. 6 32277 2016.
ISSN: ESSN 2045-2322
PubMed: 27573790
DOI: 10.1038/SREP32277