Chlorine in PDB 6njt: Mouse Endonuclease G Mutant - H97A

The structure of Mouse Endonuclease G Mutant - H97A, PDB code: 6njt was solved by C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.71 / 2.07
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	109.609, 109.609, 118.062, 90.00, 90.00, 120.00
R / Rfree (%)	18.4 / 21.3

The structure of Mouse Endonuclease G Mutant - H97A also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Chlorine atom in the Mouse Endonuclease G Mutant - H97A (pdb code 6njt). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 11 binding sites of Chlorine where determined in the Mouse Endonuclease G Mutant - H97A, PDB code: 6njt:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Chlorine binding site 1 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:37.0 occ:1.00 O A:HOH595 2.7 47.0 1.0 N A:GLU73 3.2 28.8 1.0 O A:HOH477 3.3 27.6 1.0 CA A:ARG72 3.6 27.2 0.6 CA A:ARG72 3.6 27.2 0.4 ND2 A:ASN85 3.6 27.1 1.0 CG A:GLU73 3.9 27.3 1.0 C A:ARG72 3.9 30.1 1.0 CD A:ARG72 4.0 22.0 0.6 CB A:ARG72 4.2 28.7 0.6 CB A:GLU73 4.2 26.8 1.0 CB A:ARG72 4.2 28.7 0.4 O A:PHE71 4.2 26.7 1.0 OE1 A:GLU73 4.2 35.2 1.0 CL A:CL307 4.3 72.0 1.0 CA A:GLU73 4.3 28.7 1.0 O A:HOH555 4.3 31.5 1.0 CD A:GLU73 4.4 32.4 1.0 CG A:ASN85 4.7 28.9 1.0 N A:ARG72 4.7 24.2 1.0 CG A:ARG72 4.7 31.0 0.6 C A:PHE71 4.9 27.5 1.0

Chlorine binding site 2 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:36.5 occ:1.00 N A:ARG95 3.1 21.8 1.0 O A:HOH543 3.2 26.2 1.0 NE A:ARG95 3.2 29.5 1.0 O A:HOH434 3.3 31.4 1.0 NH2 A:ARG95 3.6 32.3 1.0 CB A:ARG95 3.8 21.8 1.0 CA A:ASP94 3.8 20.9 1.0 CZ A:ARG95 3.8 33.2 1.0 C A:ASP94 4.0 29.4 1.0 CA A:ARG95 4.0 24.9 1.0 CB A:ASP94 4.1 21.7 1.0 OD1 A:ASP94 4.1 23.3 1.0 CG A:ARG95 4.2 21.5 1.0 CG A:ASP94 4.2 23.0 1.0 O A:HOH458 4.2 26.2 1.0 CE2 A:PHE71 4.2 17.4 1.0 CD A:ARG95 4.3 21.1 1.0 O A:ARG95 4.5 22.4 1.0 C A:ARG95 4.6 23.4 1.0 CD2 A:PHE71 4.7 27.8 1.0

Chlorine binding site 3 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl304 b:26.1 occ:1.00 NZ B:LYS170 3.1 25.8 1.0 NZ A:LYS170 3.2 23.4 1.0 NH2 B:ARG160 3.6 28.7 1.0 NH2 A:ARG160 3.7 31.6 1.0 CD B:LYS170 3.8 19.9 1.0 CE B:LYS170 3.9 21.0 1.0 CD A:LYS170 4.1 24.0 1.0 CE A:LYS170 4.1 24.3 1.0 O A:HOH536 4.3 26.2 1.0 OE2 A:GLU162 4.5 23.0 1.0 OE2 B:GLU162 4.6 32.0 1.0 OE1 B:GLU162 4.8 27.1 1.0 O B:HOH593 4.8 57.9 1.0 OE1 A:GLU162 4.9 27.7 1.0 CZ B:ARG160 4.9 32.0 1.0 CZ A:ARG160 5.0 29.2 1.0

Chlorine binding site 4 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl305 b:36.8 occ:1.00 O A:HOH563 2.9 33.2 1.0 O A:HOH498 3.2 21.7 1.0 N A:ALA102 3.2 18.3 1.0 CB A:ALA102 3.3 32.7 1.0 O A:HOH485 3.5 41.8 1.0 CA A:ALA102 3.8 28.0 1.0 N A:ALA101 3.9 22.9 1.0 CB A:ALA101 3.9 20.1 1.0 C A:ALA101 4.1 26.0 1.0 CA A:ALA101 4.2 26.7 1.0 CB A:ALA100 4.4 16.9 1.0 C A:ALA100 4.6 23.6 1.0 O A:HOH522 4.7 21.9 1.0 O A:HOH407 4.8 19.2 1.0 CA A:ALA100 4.9 21.3 1.0

Chlorine binding site 5 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl306 b:57.6 occ:1.00 OG A:SER225 3.7 39.7 1.0 CB A:SER225 4.1 23.3 1.0 O A:SER225 4.2 27.4 1.0 CD1 A:LEU158 4.3 21.7 1.0 CG2 A:VAL204 4.4 20.8 1.0 C A:SER225 4.5 28.7 1.0 CG1 A:VAL204 4.7 15.7 1.0 O A:HOH453 4.8 37.4 1.0 CD1 A:LEU156 4.8 22.5 1.0 N A:ILE226 4.8 31.1 1.0 CB A:VAL204 4.9 23.9 1.0 CG1 A:VAL221 4.9 20.1 1.0 CG2 A:VAL221 4.9 22.1 1.0 CA A:SER225 5.0 29.9 1.0 CG1 A:ILE226 5.0 25.4 1.0

Chlorine binding site 6 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl307 b:72.0 occ:1.00 OE1 A:GLU73 4.2 35.2 1.0 O A:HOH477 4.2 27.6 1.0 CL A:CL302 4.3 37.0 1.0 O A:HOH535 4.3 31.2 1.0 O A:HOH555 4.4 31.5 1.0 O A:HOH466 4.4 34.4 1.0 O A:HOH595 4.6 47.0 1.0 CD A:GLU73 4.8 32.4 1.0 O A:HOH570 4.9 32.3 1.0

Chlorine binding site 7 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl308 b:71.7 occ:1.00 O B:HOH557 3.3 41.9 1.0 N A:LEU20 3.5 50.4 1.0 N A:GLU19 3.5 55.8 1.0 O B:HOH475 3.6 38.0 1.0 CB A:LEU20 3.7 28.8 1.0 O A:HOH461 4.0 48.1 1.0 O B:HOH573 4.1 46.0 1.0 CA A:LEU20 4.2 40.4 1.0 CA A:GLU19 4.4 52.7 1.0 C A:GLU19 4.5 44.8 1.0 O B:HOH512 4.8 30.9 1.0 CD2 A:LEU20 4.9 35.0 1.0 CG A:LEU20 4.9 37.7 1.0

Chlorine binding site 8 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl302 b:34.2 occ:1.00 O B:HOH582 3.1 52.6 1.0 O B:HOH429 3.2 25.7 1.0 N B:GLU73 3.3 20.3 1.0 ND2 B:ASN85 3.6 27.2 1.0 CA B:ARG72 3.6 21.7 1.0 C B:ARG72 4.0 21.7 1.0 CG B:GLU73 4.0 24.8 1.0 O B:HOH602 4.0 39.8 1.0 CD B:ARG72 4.1 27.2 1.0 CB B:ARG72 4.2 24.1 1.0 CB B:GLU73 4.2 22.4 1.0 OE1 B:GLU73 4.2 24.5 1.0 CA B:GLU73 4.3 28.7 1.0 O B:PHE71 4.3 22.6 1.0 O B:HOH564 4.4 32.9 1.0 CD B:GLU73 4.5 28.8 1.0 CG B:ASN85 4.7 26.5 1.0 CG B:ARG72 4.8 25.0 1.0 N B:ARG72 4.8 22.2 1.0 O B:GLU73 4.9 28.8 1.0 C B:PHE71 5.0 28.0 1.0

Chlorine binding site 9 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl303 b:41.8 occ:1.00 O B:HOH601 3.0 38.3 1.0 O B:HOH532 3.1 22.4 1.0 N B:ALA102 3.2 24.5 1.0 O B:HOH420 3.4 37.7 1.0 CB B:ALA102 3.5 24.6 1.0 CB B:ALA101 3.9 19.9 1.0 N B:ALA101 3.9 25.1 1.0 CA B:ALA102 3.9 27.7 1.0 C B:ALA101 4.1 25.7 1.0 CA B:ALA101 4.2 28.2 1.0 O B:HOH405 4.5 40.8 1.0 CB B:ALA100 4.6 24.0 1.0 O B:HOH550 4.6 22.7 1.0 C B:ALA100 4.6 25.9 1.0 O B:HOH422 4.8 21.9 1.0 CA B:ALA100 4.9 21.7 1.0 O B:HOH618 5.0 21.7 1.0

Chlorine binding site 10 out of 11 in the Mouse Endonuclease G Mutant - H97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 10 of Mouse Endonuclease G Mutant - H97A within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl304 b:40.2 occ:1.00 N B:ARG95 3.2 20.1 1.0 O B:HOH494 3.3 26.7 1.0 O B:HOH533 3.3 27.4 1.0 NE B:ARG95 3.3 21.4 1.0 NH2 B:ARG95 3.5 32.5 1.0 CA B:ASP94 3.7 19.9 1.0 CZ B:ARG95 3.9 31.7 1.0 CB B:ASP94 3.9 23.6 1.0 OD1 B:ASP94 4.0 23.1 1.0 C B:ASP94 4.0 28.0 1.0 CB B:ARG95 4.0 19.2 1.0 CG B:ASP94 4.1 25.6 1.0 CA B:ARG95 4.1 21.4 1.0 O B:HOH476 4.2 26.2 1.0 CE2 B:PHE71 4.3 26.0 1.0 CG B:ARG95 4.4 19.6 1.0 CD B:ARG95 4.4 23.1 1.0 O B:ARG95 4.6 22.8 1.0 C B:ARG95 4.7 24.4 1.0 CD2 B:PHE71 4.9 23.8 1.0 OD2 B:ASP94 4.9 24.7 1.0 O B:PHE93 5.0 22.2 1.0

C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho. Structural Adaptation of Vertebrate Endonuclease G For 5-Hydroxymethylcytosine Recognition and Function To Be Published.