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Chlorine in PDB 6rk7: Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum

Enzymatic activity of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum

All present enzymatic activity of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum:
2.5.1.6;

Protein crystallography data

The structure of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum, PDB code: 6rk7 was solved by A.Shahar, R.Zarivach, S.Bershtein, D.Kleiner, F.Shmulevich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.66 / 1.80
Space group I 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 113.999, 106.139, 234.377, 90.00, 96.86, 90.00
R / Rfree (%) 16.9 / 19.4

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum (pdb code 6rk7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 6 binding sites of Chlorine where determined in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum, PDB code: 6rk7:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6;

Chlorine binding site 1 out of 6 in 6rk7

Go back to Chlorine Binding Sites List in 6rk7
Chlorine binding site 1 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl403

b:34.1
occ:1.00
NZ A:LYS160 3.1 28.9 1.0
NE2 A:HIS16 3.2 29.4 1.0
NZ A:LYS238 3.2 32.1 1.0
CE A:LYS238 3.6 31.1 1.0
OD2 B:ASP113 3.7 31.9 1.0
CE1 A:HIS16 3.7 29.2 1.0
C5' A:SAM401 3.7 31.8 1.0
O A:HOH546 3.9 60.0 1.0
C4' A:SAM401 3.9 29.4 1.0
CE A:LYS160 4.0 28.9 1.0
O A:HOH518 4.1 44.0 1.0
OD2 A:ASP18 4.2 29.7 1.0
OD2 A:ASP231 4.2 34.7 1.0
O A:HOH522 4.3 31.0 1.0
O3' A:SAM401 4.4 31.4 1.0
CD2 A:HIS16 4.4 26.7 1.0
O A:HOH775 4.4 58.1 1.0
CD A:LYS160 4.5 27.6 1.0
C3' A:SAM401 4.8 29.9 1.0
CG B:ASP113 4.8 28.5 1.0
ND1 A:HIS16 5.0 26.8 1.0

Chlorine binding site 2 out of 6 in 6rk7

Go back to Chlorine Binding Sites List in 6rk7
Chlorine binding site 2 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl403

b:34.5
occ:1.00
NE2 B:HIS16 3.1 24.7 1.0
NZ B:LYS160 3.1 33.5 1.0
NZ B:LYS238 3.3 32.0 1.0
CE B:LYS238 3.6 31.0 1.0
CE1 B:HIS16 3.7 26.6 1.0
C5' B:SAM401 3.7 36.1 1.0
O A:HOH547 3.7 54.8 1.0
OD2 A:ASP113 3.8 35.4 1.0
O A:HOH508 3.8 53.8 1.0
CE B:LYS160 3.9 33.7 1.0
C4' B:SAM401 3.9 32.4 1.0
O B:HOH555 4.0 50.4 1.0
O B:HOH518 4.1 44.5 1.0
OD2 B:ASP231 4.2 39.5 1.0
OD2 B:ASP18 4.3 31.4 1.0
CD2 B:HIS16 4.3 25.8 1.0
O B:HOH513 4.3 34.4 1.0
O3' B:SAM401 4.3 33.5 1.0
CD B:LYS160 4.5 33.0 1.0
C3' B:SAM401 4.8 31.6 1.0
CG A:ASP113 4.8 34.3 1.0
O4' B:SAM401 4.9 31.6 1.0
ND1 B:HIS16 4.9 27.4 1.0

Chlorine binding site 3 out of 6 in 6rk7

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Chlorine binding site 3 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cl403

b:33.9
occ:1.00
O E:HOH720 3.1 52.7 1.0
NE2 E:HIS16 3.1 23.6 1.0
NZ E:LYS160 3.2 30.5 1.0
NZ E:LYS238 3.2 28.8 1.0
O E:HOH735 3.4 56.8 1.0
CE1 E:HIS16 3.6 25.6 1.0
CE E:LYS238 3.7 29.5 1.0
OD2 F:ASP113 3.7 31.8 1.0
C5' E:SAM401 3.8 35.5 1.0
CE E:LYS160 3.9 29.4 1.0
C4' E:SAM401 4.0 32.4 1.0
O E:HOH630 4.0 54.7 1.0
O E:HOH551 4.0 44.5 1.0
OD2 E:ASP18 4.2 26.4 1.0
OD2 E:ASP231 4.2 36.0 1.0
CD2 E:HIS16 4.3 25.4 1.0
O3' E:SAM401 4.3 31.0 1.0
O E:HOH509 4.4 46.2 1.0
O E:HOH510 4.4 36.6 1.0
CD E:LYS160 4.6 28.0 1.0
CG F:ASP113 4.8 33.5 1.0
C3' E:SAM401 4.9 30.1 1.0
ND1 E:HIS16 4.9 24.1 1.0

Chlorine binding site 4 out of 6 in 6rk7

Go back to Chlorine Binding Sites List in 6rk7
Chlorine binding site 4 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Cl403

b:31.6
occ:1.00
NE2 F:HIS16 3.1 26.4 1.0
NZ F:LYS160 3.1 29.8 1.0
NZ F:LYS238 3.2 30.8 1.0
CE F:LYS238 3.6 27.9 1.0
CE1 F:HIS16 3.6 28.8 1.0
C5' F:SAM401 3.7 30.0 1.0
OD2 E:ASP113 3.7 31.1 1.0
C4' F:SAM401 3.8 27.3 1.0
CE F:LYS160 3.9 29.1 1.0
O F:HOH1122 4.0 54.8 1.0
O F:HOH1118 4.1 37.6 1.0
O E:HOH511 4.1 53.1 1.0
OD2 F:ASP231 4.2 34.8 1.0
OD2 F:ASP18 4.2 26.7 1.0
CD2 F:HIS16 4.3 24.8 1.0
O3' F:SAM401 4.4 26.9 1.0
O F:HOH1106 4.4 34.4 1.0
CD F:LYS160 4.5 25.5 1.0
C3' F:SAM401 4.8 26.9 1.0
CG E:ASP113 4.8 30.6 1.0
O4' F:SAM401 4.9 26.3 1.0
ND1 F:HIS16 4.9 26.9 1.0

Chlorine binding site 5 out of 6 in 6rk7

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Chlorine binding site 5 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Cl403

b:38.1
occ:1.00
NE2 G:HIS16 3.1 32.9 1.0
NZ G:LYS160 3.1 34.0 1.0
NZ G:LYS238 3.2 37.5 1.0
CE G:LYS238 3.6 37.7 1.0
O H:HOH530 3.6 62.8 1.0
CE1 G:HIS16 3.6 33.2 1.0
OD2 H:ASP113 3.7 41.1 1.0
O H:HOH506 3.7 51.4 1.0
C5' G:SAM401 3.8 35.8 1.0
CE G:LYS160 3.8 34.0 1.0
C4' G:SAM401 4.0 34.4 1.0
O G:HOH539 4.1 51.4 1.0
OD2 G:ASP231 4.2 40.5 1.0
O G:HOH501 4.2 44.6 1.0
OD2 G:ASP18 4.2 35.6 1.0
CD2 G:HIS16 4.3 35.5 1.0
O G:HOH510 4.3 38.2 1.0
O3' G:SAM401 4.3 33.4 1.0
CD G:LYS160 4.5 33.3 1.0
CG H:ASP113 4.8 36.4 1.0
C3' G:SAM401 4.9 35.8 1.0
ND1 G:HIS16 4.9 32.6 1.0

Chlorine binding site 6 out of 6 in 6rk7

Go back to Chlorine Binding Sites List in 6rk7
Chlorine binding site 6 out of 6 in the Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Inter-Dimeric Interface Controls Function and Stability of S- Methionine Adenosyltransferase From U. Urealiticum within 5.0Å range:
probe atom residue distance (Å) B Occ
H:Cl403

b:39.9
occ:1.00
NZ H:LYS160 3.1 43.0 1.0
NE2 H:HIS16 3.1 34.5 1.0
NZ H:LYS238 3.3 39.9 1.0
CE H:LYS238 3.7 37.4 1.0
OD2 G:ASP113 3.7 45.7 1.0
CE1 H:HIS16 3.7 34.8 1.0
C5' H:SAM401 3.7 43.2 1.0
CE H:LYS160 3.9 41.3 1.0
C4' H:SAM401 3.9 42.3 1.0
O H:HOH544 4.0 47.5 1.0
OD2 H:ASP231 4.2 46.4 1.0
O3' H:SAM401 4.2 37.2 1.0
OD2 H:ASP18 4.3 36.5 1.0
O H:HOH507 4.3 39.7 1.0
CD2 H:HIS16 4.3 34.8 1.0
CD H:LYS160 4.6 39.4 1.0
CG G:ASP113 4.7 43.6 1.0
C3' H:SAM401 4.7 39.1 1.0
ND1 H:HIS16 5.0 33.0 1.0

Reference:

D.Kleiner, F.Shmulevich, R.Zarivach, A.Shahar, M.Sharon, G.Ben-Nissan, S.Bershtein. The Inter-Dimeric Interface Controls Function and Stability of Ureaplasma Urealiticum Methionine S-Adenosyltransferase. J.Mol.Biol. 2019.
ISSN: ESSN 1089-8638
PubMed: 31520601
DOI: 10.1016/J.JMB.2019.09.003
Page generated: Mon Jul 29 14:32:29 2024

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