Chlorine in PDB 7lps: Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2)

The structure of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2), PDB code: 7lps was solved by R.P.Nowak, E.S.Fischer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	150.57 / 3.78
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	151.8, 117.292, 196.713, 90, 97.3, 90
R / Rfree (%)	28.9 / 30.3

The structure of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) also contains other interesting chemical elements:

Zinc

(Zn)

8 atoms

The binding sites of Chlorine atom in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) (pdb code 7lps). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2), PDB code: 7lps:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl502 b:138.9 occ:1.00 CL B:RN9502 0.0 138.9 1.0 C19 B:RN9502 1.8 123.2 1.0 C20 B:RN9502 2.7 116.6 1.0 C17 B:RN9502 2.8 115.9 1.0 O B:PRO352 2.9 104.3 1.0 C18 B:RN9502 3.1 97.8 1.0 C B:PRO352 3.6 102.6 1.0 CB B:PRO352 3.7 98.9 1.0 CZ B:PHE102 3.9 112.3 1.0 CA B:PRO352 4.0 96.1 1.0 C14 B:RN9502 4.0 116.5 1.0 C16 B:RN9502 4.1 120.0 1.0 CE2 B:PHE102 4.3 118.2 1.0 N B:HIS353 4.5 98.8 1.0 C15 B:RN9502 4.6 125.0 1.0 NE2 B:HIS353 4.7 106.7 1.0 CE1 B:HIS353 4.8 99.1 1.0 CD2 B:HIS353 4.8 96.9 1.0 NE2 B:GLN100 4.9 107.5 1.0 ND1 B:HIS353 4.9 97.4 1.0 CA B:HIS353 5.0 88.4 1.0 CG B:HIS353 5.0 98.1 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl1002 b:152.8 occ:1.00 CL E:RN91002 0.0 152.8 1.0 C19 E:RN91002 1.8 143.7 1.0 C20 E:RN91002 2.8 142.7 1.0 C17 E:RN91002 2.8 144.5 1.0 C18 E:RN91002 3.0 127.5 1.0 C14 E:RN91002 4.0 144.1 1.0 C16 E:RN91002 4.1 151.7 1.0 CB E:PRO352 4.2 104.6 1.0 CD2 E:HIS378 4.5 108.9 1.0 CA E:PRO352 4.5 95.3 1.0 C15 E:RN91002 4.6 154.7 1.0 O E:PRO352 4.6 111.5 1.0 OE1 E:GLN100 4.7 104.7 1.0 NE2 E:HIS378 4.8 102.5 1.0 CG E:PRO352 5.0 107.6 1.0 NE2 E:GLN100 5.0 108.8 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) within 5.0Å range: probe atom residue distance (Å) B Occ H:Cl502 b:142.7 occ:1.00 CL H:RN9502 0.0 142.7 1.0 C19 H:RN9502 1.8 127.4 1.0 C20 H:RN9502 2.8 120.9 1.0 C17 H:RN9502 2.8 120.5 1.0 C18 H:RN9502 3.1 102.3 1.0 O H:PRO352 3.4 104.0 1.0 CZ H:PHE102 3.4 124.6 1.0 CE2 H:PHE102 3.6 131.0 1.0 C H:PRO352 4.0 93.6 1.0 C14 H:RN9502 4.0 121.3 1.0 C16 H:RN9502 4.1 125.0 1.0 ND1 H:HIS353 4.3 110.8 1.0 CE1 H:HIS353 4.3 108.2 1.0 CB H:PRO352 4.4 104.8 1.0 C15 H:RN9502 4.6 130.1 1.0 CE1 H:PHE102 4.6 118.7 1.0 CA H:PRO352 4.6 89.6 1.0 N H:HIS353 4.7 93.7 1.0 CA H:HIS353 4.9 103.6 1.0 NE2 H:HIS353 4.9 110.6 1.0 CD2 H:PHE102 4.9 131.8 1.0 CG H:HIS353 4.9 111.4 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of DDB1-Crbn-ALV1 Complex Bound to Helios (IKZF2 ZF2) within 5.0Å range: probe atom residue distance (Å) B Occ K:Cl1002 b:140.8 occ:1.00 CL K:RN91002 0.0 140.8 1.0 C19 K:RN91002 1.8 125.4 1.0 C17 K:RN91002 2.7 118.3 1.0 C20 K:RN91002 2.8 119.1 1.0 C18 K:RN91002 3.1 99.9 1.0 O K:PRO352 3.7 110.7 1.0 CB K:PRO352 4.0 105.9 1.0 C16 K:RN91002 4.0 122.7 1.0 C14 K:RN91002 4.0 119.4 1.0 CZ K:PHE102 4.0 117.9 1.0 C K:PRO352 4.2 104.9 1.0 CE2 K:PHE102 4.3 122.1 1.0 CA K:PRO352 4.4 94.9 1.0 C15 K:RN91002 4.5 127.9 1.0 OE1 K:GLN100 4.9 117.5 1.0

E.S.Wang, A.L.Verano, R.P.Nowak, J.C.Yuan, K.A.Donovan, N.A.Eleuteri, H.Yue, K.H.Ngo, P.H.Lizotte, P.C.Gokhale, N.S.Gray, E.S.Fischer. Acute Pharmacological Degradation of Helios Destabilizes Regulatory T Cells Nat.Chem.Biol. 2021.
ISSN: ESSN 1552-4469
DOI: 10.1038/S41589-021-00802-W