Chlorine in PDB 2o4w: T4 Lysozyme Circular Permutant

All present enzymatic activity of T4 Lysozyme Circular Permutant:
3.2.1.17;

The structure of T4 Lysozyme Circular Permutant, PDB code: 2o4w was solved by M.Llinas, S.M.Crowder, N.Echols, T.Alber, S.Marqusee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	60.118, 60.118, 96.531, 90.00, 90.00, 120.00
R / Rfree (%)	17.5 / 21.3

The binding sites of Chlorine atom in the T4 Lysozyme Circular Permutant (pdb code 2o4w). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the T4 Lysozyme Circular Permutant, PDB code: 2o4w:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the T4 Lysozyme Circular Permutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of T4 Lysozyme Circular Permutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl501 b:19.8 occ:1.00 O A:HOH659 2.6 47.7 1.0 O A:GLY30 2.7 13.9 1.0 O A:PHE104 2.7 13.6 1.0 O A:HOH527 2.7 27.6 1.0 O A:HOH586 2.8 27.2 1.0 O A:HOH515 2.9 17.4 1.0 O A:HOH529 3.0 19.2 1.0 CE2 A:PHE104 3.3 13.0 1.0 CD2 A:PHE104 3.3 11.9 1.0 C A:PHE104 3.8 14.2 1.0 O A:HOH589 3.8 29.8 1.0 O A:HOH503 3.8 28.7 1.0 C A:GLY30 3.9 14.0 1.0 CZ A:PHE104 4.0 14.6 1.0 CG A:PHE104 4.1 13.3 1.0 O A:HOH564 4.5 25.9 1.0 CA A:PHE104 4.6 13.9 1.0 N A:GLN105 4.6 14.5 1.0 CA A:GLN105 4.6 15.2 1.0 O A:HOH526 4.6 31.2 1.0 N A:LEU32 4.7 16.5 1.0 CE1 A:PHE104 4.7 14.3 1.0 CA A:GLY30 4.7 13.9 1.0 OE2 A:GLU181 4.7 21.8 1.0 CD1 A:PHE104 4.7 11.2 1.0 O A:HOH547 4.8 32.0 1.0 O A:GLN105 4.8 14.4 1.0 CB A:PHE104 4.9 13.5 1.0 N A:HIS31 4.9 14.2 1.0 CA A:HIS31 5.0 14.6 1.0

Chlorine binding site 2 out of 2 in the T4 Lysozyme Circular Permutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of T4 Lysozyme Circular Permutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl502 b:26.5 occ:1.00 O A:HOH520 3.0 25.3 1.0 ND2 A:ASN132 3.2 16.7 1.0 NZ A:LYS135 3.3 24.2 1.0 CB A:ASN132 3.6 16.1 1.0 CA A:ASN132 3.7 15.9 1.0 CG A:ASN132 3.9 17.0 1.0 CE A:LYS135 3.9 23.3 1.0 N A:ASN132 4.0 15.9 1.0 CD A:LYS135 4.0 20.6 1.0 CG1 A:VAL131 4.1 18.7 1.0 C A:VAL131 4.5 17.8 1.0 CE A:MET120 4.7 20.7 1.0 O A:VAL131 4.7 17.0 1.0 O A:GLU128 4.7 16.2 1.0 O A:HOH653 4.8 52.5 1.0 CB A:VAL131 4.9 18.2 1.0

J.Cellitti, M.Llinas, N.Echols, E.A.Shank, B.Gillespie, E.Kwon, S.M.Crowder, F.W.Dahlquist, T.Alber, S.Marqusee. Exploring Subdomain Cooperativity in T4 Lysozyme I: Structural and Energetic Studies of A Circular Permutant and Protein Fragment. Protein Sci. V. 16 842 2007.
ISSN: ISSN 0961-8368
PubMed: 17400926
DOI: 10.1110/PS.062628607