Chlorine in PDB 2v4a: Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii.

The structure of Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii., PDB code: 2v4a was solved by M.K.Koski, R.Hieta, C.Bollner, K.I.Kivirikko, J.Myllyharju, R.K.Wierenga, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.87 / 1.93
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	137.490, 137.490, 88.130, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 22.3

The binding sites of Chlorine atom in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii. (pdb code 2v4a). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii., PDB code: 2v4a:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii. within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1250 b:29.0 occ:1.00 O A:HOH2089 2.8 41.2 1.0 OG1 A:THR178 3.0 27.3 1.0 O A:HOH2049 3.1 24.1 1.0 NZ A:LYS237 3.1 21.5 1.0 CB A:THR178 3.8 23.4 1.0 CG2 A:THR178 3.8 24.9 1.0 CE A:LYS237 3.8 24.3 1.0 CA A:THR178 4.0 23.7 1.0 CB A:SER229 4.0 26.4 1.0 CD1 A:LEU166 4.0 36.7 1.0 OG A:SER229 4.0 26.7 1.0 O A:GLY228 4.2 26.5 1.0 CA A:SER229 4.2 26.2 1.0 CD2 A:LEU166 4.2 35.5 1.0 OH A:TYR134 4.3 24.6 1.0 N A:THR178 4.5 21.8 1.0 C A:GLY228 4.5 26.1 1.0 N A:SER229 4.5 26.1 1.0 CG A:LEU166 4.6 31.9 1.0 O A:HOH2094 4.6 33.0 1.0 CD2 A:HIS227 4.7 29.5 1.0 CZ A:TYR134 4.9 23.3 1.0 CE1 A:TYR168 5.0 24.3 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii. within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1250 b:28.2 occ:1.00 O B:HOH2041 2.9 31.9 1.0 OG1 B:THR178 3.0 27.4 1.0 NZ B:LYS237 3.1 24.8 1.0 CD1 B:LEU166 3.8 25.0 1.0 CB B:THR178 3.8 25.7 1.0 CE B:LYS237 3.9 24.1 1.0 CG2 B:THR178 4.0 25.7 1.0 CB B:SER229 4.0 25.8 1.0 CA B:THR178 4.0 25.0 1.0 O B:GLY228 4.1 26.3 1.0 OG B:SER229 4.2 28.7 1.0 CA B:SER229 4.2 26.3 1.0 OH B:TYR134 4.2 26.5 1.0 CD2 B:LEU166 4.3 27.1 1.0 CD2 B:HIS227 4.3 29.4 1.0 C B:GLY228 4.5 26.4 1.0 CG B:LEU166 4.5 27.5 1.0 N B:SER229 4.5 26.4 1.0 N B:THR178 4.5 22.8 1.0 O B:HOH2084 4.6 37.0 1.0 NE2 B:HIS227 4.8 29.6 1.0 CZ B:TYR134 4.9 24.5 1.0 CE1 B:TYR168 5.0 27.8 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii. within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1250 b:35.7 occ:1.00 O C:HOH2037 3.0 26.2 1.0 OG1 C:THR178 3.1 25.8 1.0 NZ C:LYS237 3.2 26.0 1.0 CB C:SER229 3.7 27.9 1.0 CE C:LYS237 3.8 25.2 1.0 CD1 C:LEU166 3.9 26.6 1.0 CB C:THR178 3.9 24.1 1.0 OH C:TYR134 4.0 26.6 1.0 CA C:THR178 4.0 24.0 1.0 CG2 C:THR178 4.0 22.8 1.0 CA C:SER229 4.1 27.7 1.0 O C:GLY228 4.2 28.3 1.0 OG C:SER229 4.2 26.0 1.0 CD2 C:LEU166 4.3 25.1 1.0 N C:SER229 4.4 28.4 1.0 C C:GLY228 4.5 28.3 1.0 CG C:LEU166 4.6 26.1 1.0 N C:THR178 4.6 23.3 1.0 O C:HOH2066 4.6 28.9 1.0 CZ C:TYR134 4.6 26.0 1.0 CE1 C:TYR134 4.9 26.0 1.0 CE1 C:TYR168 4.9 25.1 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of the Semet-Labeled Prolyl-4 Hydroxylase (P4H) Type I From Green Algae Chlamydomonas Reinhardtii. within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl1250 b:45.0 occ:1.00 OG1 D:THR178 2.9 27.6 1.0 NZ D:LYS237 3.1 19.5 1.0 O D:HOH2029 3.1 32.6 1.0 CD1 D:LEU166 3.6 26.0 1.0 CB D:THR178 3.6 25.6 1.0 CA D:THR178 3.8 25.6 1.0 CB D:SER229 3.8 27.3 1.0 CG2 D:THR178 3.8 23.9 1.0 CE D:LYS237 3.9 25.4 1.0 O D:GLY228 3.9 27.0 1.0 OH D:TYR134 4.1 31.9 1.0 CA D:SER229 4.2 27.5 1.0 OG D:SER229 4.3 26.0 1.0 CD2 D:HIS227 4.4 32.2 1.0 C D:GLY228 4.4 27.6 1.0 N D:THR178 4.4 25.5 1.0 CD2 D:LEU166 4.4 24.5 1.0 N D:SER229 4.5 27.6 1.0 CG D:LEU166 4.6 25.1 1.0 CZ D:TYR134 4.6 30.3 1.0 O D:HOH2056 4.8 38.0 1.0 CE1 D:TYR134 4.9 32.0 1.0 CE1 D:TYR168 4.9 24.2 1.0 NE2 D:HIS227 5.0 32.9 1.0

M.K.Koski, R.Hieta, C.Bollner, K.I.Kivirikko, J.Myllyharju, R.K.Wierenga. The Active Site of An Algal Prolyl 4-Hydroxylase Has A Large Structural Plasticity. J.Biol.Chem. V. 282 37112 2007.
ISSN: ISSN 0021-9258
PubMed: 17940281
DOI: 10.1074/JBC.M706554200