Atomistry » Chlorine » PDB 2xep-2xmb » 2xfg
Atomistry »
  Chlorine »
    PDB 2xep-2xmb »
      2xfg »

Chlorine in PDB 2xfg: Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules

Enzymatic activity of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules

All present enzymatic activity of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules:
3.2.1.4;

Protein crystallography data

The structure of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules, PDB code: 2xfg was solved by S.Petkun, R.Lamed, S.Jindou, T.Burstein, O.Yaniv, Y.Shoham, J.W.L.Shimon, E.A.Bayer, F.Frolow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.868 / 1.68
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 70.399, 88.541, 106.486, 90.00, 90.00, 90.00
R / Rfree (%) 14.14 / 17.59

Other elements in 2xfg:

The structure of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules also contains other interesting chemical elements:

Calcium (Ca) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules (pdb code 2xfg). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules, PDB code: 2xfg:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 2xfg

Go back to Chlorine Binding Sites List in 2xfg
Chlorine binding site 1 out of 3 in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1448

b:24.4
occ:1.00
 HZ1 A:LYS441 2.4 48.6 1.0
H A:ALA84 2.7 21.1 0.6
HB3 A:ASP83 2.8 27.9 0.6
H A:ALA84 2.8 21.1 0.4
HD2 A:LYS441 2.8 35.2 1.0
HA A:TYR81 2.8 23.1 1.0
HB2 A:ASP83 2.9 29.4 0.4
HD1 A:TYR81 3.1 23.6 1.0
NZ A:LYS441 3.2 40.5 1.0
O A:HOH2696 3.3 32.0 1.0
H A:ASP83 3.3 24.6 0.4
HE3 A:LYS441 3.3 43.5 1.0
O A:HOH2196 3.3 28.7 1.0
H A:ASP83 3.3 24.1 0.6
HB1 A:ALA84 3.4 25.0 1.0
N A:ALA84 3.4 17.6 1.0
O A:HOH2187 3.5 43.3 1.0
CE A:LYS441 3.5 36.3 1.0
CD A:LYS441 3.6 29.4 1.0
CA A:TYR81 3.6 19.3 1.0
HB3 A:TYR81 3.6 20.9 1.0
HZ3 A:LYS441 3.6 48.6 1.0
CB A:ASP83 3.6 23.3 0.6
C A:TYR81 3.8 18.9 1.0
HZ2 A:LYS441 3.8 48.6 1.0
CB A:ASP83 3.8 24.5 0.4
N A:ASP83 3.8 20.5 0.4
N A:ASP83 3.8 20.1 0.6
O A:TYR81 4.0 15.7 1.0
HB2 A:ASP83 4.0 27.9 0.6
CD1 A:TYR81 4.0 19.7 1.0
CA A:ASP83 4.0 21.0 0.6
CB A:TYR81 4.0 17.5 1.0
CA A:ASP83 4.1 21.4 0.4
CB A:ALA84 4.1 20.9 1.0
C A:ASP83 4.1 21.5 0.4
C A:ASP83 4.1 21.8 0.6
O A:HOH2686 4.1 78.2 1.0
HB3 A:ALA84 4.1 25.0 1.0
CA A:ALA84 4.2 18.1 1.0
HD3 A:LYS441 4.2 35.2 1.0
N A:GLU82 4.3 17.7 1.0
HG3 A:LYS441 4.3 27.7 1.0
HB3 A:ASP83 4.3 29.4 0.4
HB2 A:LYS441 4.4 24.0 1.0
H A:GLU82 4.4 21.2 1.0
HE2 A:LYS441 4.5 43.5 1.0
CG A:LYS441 4.5 23.1 1.0
O A:GLU80 4.5 16.2 1.0
HA A:ALA84 4.5 21.7 1.0
CG A:TYR81 4.5 19.1 1.0
OD2 A:ASP83 4.7 42.4 0.4
OD2 A:ASP83 4.7 26.6 0.6
CG A:ASP83 4.7 33.3 0.4
CG A:ASP83 4.7 23.9 0.6
C A:GLU82 4.8 18.9 1.0
N A:TYR81 4.8 15.0 1.0
HB2 A:ALA84 4.9 25.0 1.0
HB2 A:TYR81 4.9 20.9 1.0

Chlorine binding site 2 out of 3 in 2xfg

Go back to Chlorine Binding Sites List in 2xfg
Chlorine binding site 2 out of 3 in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1449

b:27.9
occ:1.00
 HE21 A:GLN11 2.3 22.5 1.0
HE2 A:HIS93 2.8 23.2 1.0
NE2 A:GLN11 3.1 18.8 1.0
O B:HOH2042 3.1 20.3 1.0
HA B:PRO451 3.1 28.6 1.0
O B:HOH2034 3.2 33.4 0.8
OE1 A:GLN90 3.3 22.8 1.0
HD2 A:HIS93 3.4 19.3 1.0
NE2 A:HIS93 3.4 19.4 1.0
HE22 A:GLN11 3.5 22.5 1.0
HG3 A:GLN11 3.5 16.7 1.0
O B:HOH2038 3.6 45.7 1.0
O A:HOH2214 3.6 28.7 0.8
HE22 A:GLN90 3.6 14.6 1.0
CD2 A:HIS93 3.7 16.1 1.0
HH11 A:ARG31 4.0 20.5 1.0
CA B:PRO451 4.1 23.9 1.0
CD A:GLN11 4.1 17.1 1.0
HH12 A:ARG31 4.2 20.5 1.0
CD A:GLN90 4.2 17.2 1.0
HB3 B:PRO451 4.2 30.3 1.0
CG A:GLN11 4.2 13.9 1.0
NE2 A:GLN90 4.2 12.2 1.0
O A:GLY89 4.3 17.2 1.0
NH1 A:ARG31 4.4 17.1 1.0
O B:PRO451 4.4 27.6 1.0
HG2 B:PRO449 4.6 17.8 1.0
CB B:PRO451 4.6 25.2 1.0
CE1 A:HIS93 4.7 23.4 1.0
HB3 B:PHE453 4.7 19.8 1.0
C B:PRO451 4.7 24.2 1.0
HG2 A:GLN11 4.7 16.7 1.0
HB2 B:PRO451 4.8 30.3 1.0
O B:ASP450 4.8 20.4 1.0
HD2 B:PHE453 4.8 18.3 1.0

Chlorine binding site 3 out of 3 in 2xfg

Go back to Chlorine Binding Sites List in 2xfg
Chlorine binding site 3 out of 3 in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1450

b:20.5
occ:1.00
 H A:SER208 2.4 19.7 1.0
HD21 A:ASN207 2.5 21.0 1.0
HA A:ASN207 3.1 16.8 1.0
N A:SER208 3.2 16.5 1.0
O A:HOH2387 3.2 27.3 1.0
HB3 A:ASN207 3.2 19.6 1.0
O A:HOH2386 3.2 23.9 1.0
HD2 A:TYR199 3.3 21.8 1.0
ND2 A:ASN207 3.3 17.6 1.0
HB3 A:SER208 3.5 18.3 1.0
O A:HOH2401 3.6 32.6 0.9
CA A:ASN207 3.7 14.0 1.0
CB A:ASN207 3.8 16.4 1.0
HD22 A:ASN207 3.9 21.0 1.0
HE2 A:TYR199 3.9 15.8 1.0
C A:ASN207 3.9 14.9 1.0
O A:SER208 4.0 18.5 1.0
CG A:ASN207 4.0 17.9 1.0
CD2 A:TYR199 4.1 18.2 1.0
CA A:SER208 4.1 15.5 1.0
CB A:SER208 4.1 15.3 1.0
HB2 A:SER208 4.3 18.3 1.0
CE2 A:TYR199 4.4 13.2 1.0
O A:TYR199 4.4 16.7 1.0
C A:SER208 4.5 14.5 1.0
HB3 A:ASP196 4.6 22.8 1.0
HB2 A:ASN207 4.7 19.6 1.0
HB3 A:TYR199 4.9 16.8 1.0
O A:HOH2380 4.9 39.7 1.0
O A:HOH2392 5.0 15.9 0.9
HA A:SER208 5.0 18.5 1.0
O A:HOH2169 5.0 21.7 1.0
O A:HOH2167 5.0 34.1 1.0

Reference:

S.Petkun, I.R.Grinberg, R.Lamed, S.Jindou, T.Burstein, O.Yaniv, Y.Shoham, J.W.L.Shimon, E.A.Bayer, F.Frolow. Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Its Component Parts: Structural and Functional Significance of Intermodular Linker Peerj V. 3 E1126 2015.
ISSN: ISSN 2167-8359
PubMed: 26401442
DOI: 10.7717/PEERJ.1126
Page generated: Sat Jul 20 13:49:29 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy