Chlorine in PDB 4zze: Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose

The structure of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose, PDB code: 4zze was solved by F.Fredslund, M.Ejby, J.M.Andersen, D.J.Slotboom, M.A.Hachem, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.11 / 1.76
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.880, 91.330, 142.050, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 20.5

The structure of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose also contains other interesting chemical elements:

Magnesium

(Mg)

9 atoms

The binding sites of Chlorine atom in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose (pdb code 4zze). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose, PDB code: 4zze:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl509 b:26.2 occ:1.00 HH22 A:ARG381 2.5 23.3 1.0 H A:PHE372 2.6 19.3 1.0 HA A:TYR371 3.0 18.6 1.0 HD1 A:PHE372 3.0 28.4 1.0 O A:HOH684 3.2 26.6 1.0 HH12 A:ARG381 3.2 26.8 1.0 NH2 A:ARG381 3.3 19.4 1.0 N A:PHE372 3.4 16.1 1.0 HB2 A:PHE372 3.5 19.0 1.0 HD1 A:TYR371 3.8 29.1 1.0 HH21 A:ARG381 3.9 23.3 1.0 NH1 A:ARG381 3.9 22.3 1.0 CA A:TYR371 3.9 15.5 1.0 CD1 A:PHE372 3.9 23.7 1.0 CZ A:ARG381 4.1 22.7 1.0 O A:THR370 4.1 18.1 1.0 CD1 A:TYR371 4.1 24.2 1.0 C A:TYR371 4.2 16.0 1.0 CB A:PHE372 4.2 15.8 1.0 O A:HOH838 4.2 27.9 1.0 CA A:PHE372 4.3 15.0 1.0 O A:PHE372 4.4 15.1 1.0 CG A:PHE372 4.6 17.7 1.0 CG A:TYR371 4.6 16.0 1.0 HH11 A:ARG381 4.7 26.8 1.0 CB A:TYR371 4.7 17.6 1.0 CE1 A:TYR371 4.7 23.6 1.0 HE1 A:TYR371 4.8 28.3 1.0 HB2 A:TYR371 4.8 21.1 1.0 O A:HOH689 4.8 38.0 1.0 N A:TYR371 4.9 16.5 1.0 C A:THR370 4.9 22.2 1.0 C A:PHE372 4.9 12.6 1.0 HE1 A:PHE372 4.9 19.8 1.0 CE1 A:PHE372 5.0 16.5 1.0

Chlorine binding site 2 out of 4 in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl510 b:27.9 occ:1.00 H A:GLU137 2.4 14.8 1.0 HG3 A:GLU137 2.7 37.2 1.0 HA A:ASN136 3.1 19.5 1.0 N A:GLU137 3.2 12.4 1.0 HB2 A:GLU137 3.3 27.8 1.0 O A:HOH781 3.4 31.4 1.0 CG A:GLU137 3.6 31.0 1.0 CB A:GLU137 3.8 23.1 1.0 CG A:LYS368 3.9 25.0 1.0 CA A:ASN136 3.9 16.2 1.0 HA A:LYS368 4.0 21.1 1.0 C A:ASN136 4.1 14.8 1.0 CA A:GLU137 4.1 16.5 1.0 CD A:GLU137 4.2 39.9 1.0 NZ A:LYS368 4.2 34.6 1.0 HG2 A:GLU137 4.3 37.2 1.0 O A:LEU135 4.3 12.3 1.0 CE A:LYS368 4.4 33.0 1.0 H A:GLY138 4.4 20.7 1.0 OD1 A:ASN136 4.6 16.8 1.0 CD A:LYS368 4.7 29.2 1.0 CB A:LYS368 4.7 19.6 1.0 OE1 A:GLU137 4.7 42.1 1.0 HB3 A:GLU137 4.7 27.8 1.0 OE2 A:GLU137 4.8 45.5 1.0 N A:ASN136 4.8 14.4 1.0 HA A:GLU137 4.8 19.8 1.0 CA A:LYS368 4.8 17.6 1.0 CG A:ASN136 4.8 19.1 1.0 O A:HOH1008 4.9 40.5 1.0 O A:HOH996 4.9 43.4 1.0 CB A:ASN136 4.9 17.1 1.0 C A:LEU135 4.9 16.4 1.0

Chlorine binding site 3 out of 4 in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl508 b:31.3 occ:1.00 H B:PHE372 2.4 22.3 1.0 HH22 B:ARG381 2.5 31.0 1.0 HA B:TYR371 3.0 25.5 1.0 HD1 B:PHE372 3.0 35.8 1.0 O B:HOH766 3.2 25.7 1.0 N B:PHE372 3.3 18.6 1.0 NH2 B:ARG381 3.4 25.8 1.0 O B:HOH784 3.4 37.0 1.0 HB2 B:PHE372 3.5 25.2 1.0 HD1 B:TYR371 3.5 29.7 1.0 HH12 B:ARG381 3.7 38.9 1.0 HH21 B:ARG381 3.8 31.0 1.0 CA B:TYR371 3.8 21.2 1.0 CD1 B:PHE372 3.9 29.9 1.0 CD1 B:TYR371 3.9 24.8 1.0 C B:TYR371 4.1 24.4 1.0 O B:THR370 4.1 31.5 1.0 O B:HOH809 4.2 32.1 1.0 CB B:PHE372 4.2 21.0 1.0 CA B:PHE372 4.2 21.3 1.0 O B:PHE372 4.3 18.6 1.0 CZ B:ARG381 4.3 28.0 1.0 NH1 B:ARG381 4.4 32.5 1.0 CG B:TYR371 4.5 24.0 1.0 CE1 B:TYR371 4.5 28.4 1.0 HE1 B:TYR371 4.5 34.1 1.0 CG B:PHE372 4.6 20.3 1.0 CB B:TYR371 4.6 22.0 1.0 O B:HOH1045 4.7 31.8 1.0 O B:HOH728 4.7 27.4 1.0 C B:PHE372 4.8 21.7 1.0 HB2 B:TYR371 4.8 26.4 1.0 N B:TYR371 4.8 21.9 1.0 C B:THR370 4.8 27.7 1.0 HE1 B:PHE372 4.9 34.6 1.0 CE1 B:PHE372 4.9 28.8 1.0

Chlorine binding site 4 out of 4 in the Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Raffinose and Panose Binding Protein From Bifidobacterium Animalis Subsp. Lactis Bl-04, Bound with Panose within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl509 b:28.1 occ:1.00 H B:GLU137 2.4 19.3 1.0 HG3 B:GLU137 3.0 28.4 1.0 HG2 B:LYS368 3.0 34.0 1.0 O B:HOH961 3.1 30.1 1.0 HA B:ASN136 3.1 22.0 1.0 HB2 B:GLU137 3.2 28.4 1.0 N B:GLU137 3.3 16.1 1.0 HE2 B:LYS368 3.4 26.9 1.0 CG B:GLU137 3.8 23.7 1.0 CB B:GLU137 3.8 23.6 1.0 HD3 B:LYS368 3.9 37.5 1.0 CG B:LYS368 3.9 28.3 1.0 CA B:ASN136 4.0 18.4 1.0 HA B:LYS368 4.1 31.2 1.0 C B:ASN136 4.1 13.8 1.0 CA B:GLU137 4.2 16.6 1.0 HB3 B:LYS368 4.2 32.6 1.0 CD B:LYS368 4.2 31.3 1.0 CE B:LYS368 4.2 22.4 1.0 CD B:GLU137 4.3 41.7 1.0 H B:GLY138 4.4 18.1 1.0 O B:LEU135 4.5 14.3 1.0 CB B:LYS368 4.5 27.2 1.0 O B:HOH1038 4.5 37.1 1.0 HG2 B:GLU137 4.6 28.4 1.0 OE1 B:GLU137 4.6 40.3 1.0 HG3 B:LYS368 4.6 34.0 1.0 OD1 B:ASN136 4.6 16.0 1.0 HB3 B:GLU137 4.7 28.4 1.0 HE3 B:LYS368 4.7 26.9 1.0 HA B:GLU137 4.8 19.9 1.0 N B:ASN136 4.8 14.2 1.0 CA B:LYS368 4.8 26.0 1.0 OE2 B:GLU137 4.9 45.1 1.0 CG B:ASN136 4.9 20.8 1.0

M.Ejby, F.Fredslund, J.M.Andersen, A.Vujicic Zagar, J.R.Henriksen, T.L.Andersen, B.Svensson, D.J.Slotboom, M.Abou Hachem. An Atp Binding Cassette Transporter Mediates the Uptake of Alpha-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates. J. Biol. Chem. V. 291 20220 2016.
ISSN: ESSN 1083-351X
PubMed: 27502277
DOI: 10.1074/JBC.M116.746529