Chlorine in PDB 2o24: Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex

The structure of Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex, PDB code: 2o24 was solved by G.Garau, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.68 / 1.45
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.989, 62.979, 69.390, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / 19.2

The binding sites of Chlorine atom in the Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex (pdb code 2o24). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex, PDB code: 2o24:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1240 b:12.3 occ:1.00 OH A:TYR1203 3.1 13.1 1.0 N A:VAL1068 3.2 9.7 1.0 O A:HOH1252 3.3 19.8 1.0 C A:VAL1068 3.4 9.4 1.0 C1 A:CRO1066 3.4 8.7 1.0 N2 A:CRO1066 3.4 8.4 1.0 O A:VAL1068 3.5 9.0 1.0 N A:GLN1069 3.6 9.5 1.0 N3 A:CRO1066 3.6 8.0 1.0 CA2 A:CRO1066 3.7 8.2 1.0 C2 A:CRO1066 3.8 8.2 1.0 CA A:GLN1069 3.8 9.9 1.0 CE2 A:TYR1203 3.8 11.2 1.0 CA A:VAL1068 3.8 9.5 1.0 CG A:GLN1069 3.8 10.5 1.0 CD1 A:LEU1042 3.9 13.9 1.0 CG2 A:VAL1068 3.9 10.1 1.0 CB1 A:CRO1066 3.9 10.2 1.0 CZ A:TYR1203 3.9 11.2 1.0 CA1 A:CRO1066 3.9 9.1 1.0 C3 A:CRO1066 4.2 12.3 1.0 CB A:GLN1069 4.4 9.8 1.0 CA3 A:CRO1066 4.4 10.4 1.0 OE1 A:GLU1222 4.5 16.8 1.0 CB2 A:CRO1066 4.5 9.7 1.0 OG1 A:CRO1066 4.5 11.1 1.0 CB A:VAL1068 4.5 9.2 1.0 O2 A:CRO1066 4.5 8.9 1.0 CG2 A:VAL1224 4.9 15.0 1.0 CD A:GLN1069 5.0 10.4 1.0 C A:GLN1069 5.0 10.2 1.0

Chlorine binding site 2 out of 3 in the Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1241 b:12.4 occ:1.00 N A:LEU1207 3.3 10.2 1.0 O A:HOH1377 3.3 22.0 1.0 N A:TYR1145 3.4 11.1 1.0 O A:HOH1307 3.5 20.9 1.0 CA A:ASN1144 3.6 10.3 1.0 CB A:ASN1144 3.7 10.8 1.0 CA A:ALA1206 3.8 10.8 1.0 C A:ASN1144 3.9 10.8 1.0 O A:LEU1207 4.0 11.1 1.0 O A:TYR1145 4.0 14.3 1.0 C A:ALA1206 4.1 10.4 1.0 CB A:LEU1207 4.1 10.6 1.0 CA A:LEU1207 4.2 10.3 1.0 CG A:LEU1207 4.2 11.2 1.0 O A:SER1205 4.2 12.7 1.0 CA A:TYR1145 4.4 11.7 1.0 CB A:TYR1145 4.6 11.2 1.0 C A:LEU1207 4.6 10.2 1.0 CB A:ALA1206 4.6 11.1 1.0 C A:TYR1145 4.6 13.3 1.0 O A:TYR1143 4.7 10.8 1.0 N A:ASN1144 4.8 9.9 1.0 N A:ALA1206 4.8 11.1 1.0 C A:SER1205 4.9 11.7 1.0

Chlorine binding site 3 out of 3 in the Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Spectroscopic and Structural Study of the Heterotropic Linkage Between Halide and Proton Ion Binding to Gfp Proteins: E2(Gfp)-Cl Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1242 b:17.6 occ:1.00 O A:HOH1275 2.9 6.2 1.0 O A:HOH1279 2.9 28.2 1.0 O A:HOH1271 3.1 13.5 1.0 N A:TRP1057 3.2 8.3 1.0 O A:HOH1338 3.3 22.5 1.0 CD1 A:TRP1057 3.7 8.5 1.0 CA A:PRO1056 3.7 9.3 1.0 CB A:TRP1057 3.8 8.3 1.0 CD A:PRO1058 3.9 8.8 1.0 C A:PRO1056 4.0 8.7 1.0 CD1 A:TYR1143 4.1 10.0 1.0 CA A:TRP1057 4.1 8.1 1.0 O A:HOH1416 4.1 27.5 1.0 CG A:TRP1057 4.2 8.2 1.0 O A:HOH1270 4.2 10.8 1.0 CB A:PRO1056 4.2 9.8 1.0 CE1 A:TYR1143 4.7 10.5 1.0 N A:PRO1058 4.9 8.4 1.0 NE1 A:TRP1057 4.9 8.7 1.0 O A:VAL1055 4.9 10.3 1.0

D.Arosio, G.Garau, F.Ricci, L.Marchetti, R.Bizzarri, F.Beltram. Spectroscopic and Structural Study of Proton and Halide Ion Cooperative Binding to Gfp. Biophys.J. V. 93 232 2007.
ISSN: ISSN 0006-3495
PubMed: 17434942
DOI: 10.1529/BIOPHYSJ.106.102319